Institute of Animal Breeding & Genetic, Sichuan Agricultural University, Ya'an, Sichuan, China.
J Anim Physiol Anim Nutr (Berl). 2014 Jun;98(3):522-9. doi: 10.1111/jpn.12104. Epub 2013 Aug 20.
Follistatin (FST) acts as a positive regulator of muscle development by inhibiting the activities and expression of myostatin. The recombinant duck FST protein was injected into hatching eggs and was also added to the medium of duck myoblast to study its role on duck embryonic muscle development and gene expressions. Duck embryo weight increased 3.49% (p > 0.05) in FST treatment group as compared with control group, but minor effects were found on leg or breast muscle weights of ducklings at 2 days post-hatching (p > 0.05). Relative expression of Pax7 was upregulated in both leg and breast muscle tissues (p < 0.05), while MyoD was only upregulated in leg muscle (p < 0.05), and Myf5 was only upregulated in breast muscle (p < 0.05). Relative expression of myostatin was downregulated in both muscle tissues researched (p < 0.05). In vitro studies also showed some maker genes relevant to protein synthesis and degradation, cells' proliferation and differentiation had significant changes in myoblasts after treated with FST. These results suggested that in ovo feeding of recombinant FST protein to duck hatching eggs had an effect on duck embryo development but have less roles on the duck embryonic muscle development.
卵泡抑素（Follistatin，FST）通过抑制肌肉生长抑制素（Myostatin，MSTN）的活性和表达，起到肌肉发育正向调控因子的作用。本研究将重组鸭 Follistatin 蛋白注射入孵化蛋中，同时添加到鸭成肌细胞培养液中，研究其对鸭胚肌肉发育和基因表达的影响。与对照组相比，FST 处理组鸭胚重增加了 3.49%（p＞0.05），但对出壳后 2 天雏鸭腿肌和胸肌重影响较小（p＞0.05）。Pax7 在腿肌和胸肌组织中的相对表达均上调（p＜0.05），MyoD 仅在腿肌中上调（p＜0.05），Myf5 仅在胸肌中上调（p＜0.05）。两种肌肉组织中 MSTN 的相对表达均下调（p＜0.05）。体外研究还表明，FST 处理后成肌细胞中与蛋白质合成和降解、细胞增殖和分化相关的一些标记基因发生显著变化。这些结果表明，向孵化蛋中注射重组 Follistatin 蛋白对鸭胚发育有影响，但对鸭胚肌肉发育的作用较小。
