CDX2 在 Caco-2 细胞分化中的作用。
The role of CDX2 in Caco-2 cell differentiation.
机构信息
Istituto di Biologia Cellulare e Neurobiologia, CNR, Rome, Italy.
出版信息
Eur J Pharm Biopharm. 2013 Sep;85(1):20-5. doi: 10.1016/j.ejpb.2013.03.018.
BACKGROUND
CDX2 plays a key part in the differentiation of Caco-2 cells, a colon carcinoma derived cell line that undergoes spontaneous differentiation. The effect of CDX2 expression in Caco-2 cells over time in culture has not been studied yet on a genome-wide level.
METHODS
The impact of CDX2 expression on the genomic profile of Caco-2 cells was studied by transducing cells with CDX2 targeting shRNAs. Knockdown efficiency was assessed on mRNA level and protein level by RTPCR, microarrays, and Western blots. Gene set enrichment analysis was performed to assess regulation of specific gene sets.
RESULTS
CDX2 expression had an inhibitory effect on the transcriptional activity of β-catenin/TCF at early stages of culturing, while at later stages, its role in the trans-activation of target genes specific for small intestinal enterocytes seemed more dominant.
CONCLUSIONS
The unique induction of a small intestinal signature upon differentiation in Caco-2 cells seems to be at least partially under the control of CDX2.
背景
CDX2 在结肠癌细胞系 Caco-2 的分化中起着关键作用，该细胞系会自发分化。然而，CDX2 表达在 Caco-2 细胞培养过程中的时间变化对基因组范围的影响尚未得到研究。
方法
通过转导靶向 CDX2 的 shRNA 来研究 CDX2 表达对 Caco-2 细胞基因组图谱的影响。通过 RT-PCR、微阵列和 Western blot 评估 mRNA 水平和蛋白质水平的敲低效率。进行基因集富集分析以评估特定基因集的调控。
结果
CDX2 表达在培养的早期阶段对 β-连环蛋白/TCF 的转录活性具有抑制作用，而在后期阶段，其对小肠肠细胞特异性靶基因的反式激活作用似乎更为突出。
结论
在 Caco-2 细胞分化过程中诱导的独特小肠特征似乎至少部分受到 CDX2 的控制。
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