[一种用于测定甲状腺球蛋白自身抗体的放射免疫方法的研发]
[The development of a radioimmunological method for determining autoantibodies to thyroglobulin].
作者信息
Shcherban' A I, Cherkesova T S, Bogdanovich A F, Vasilevskiĭ V I, Demidchik E P, Minaĭlo T I
出版信息
Probl Endokrinol (Mosk). 1990 May-Jun;36(3):72-5.
A radioimmunoassay to determine a titer of autoantibodies to thyroglobulin in human blood serum was developed. The scheme of analysis included a reaction between 125I-thyroglobulin and autoantibodies to thyroglobulin with subsequent separation of the produced immune complex and free polyethylene glycol-labeled thyroglobulin. A high specificity of the radioimmunoassay is ensured by the use of highly purified thyroglobulin for obtaining the labeled agent. A possibility of the use of the method for the diagnosis of autoimmune thyroid diseases was shown.
开发了一种用于测定人血清中甲状腺球蛋白自身抗体滴度的放射免疫分析方法。分析方案包括¹²⁵I-甲状腺球蛋白与甲状腺球蛋白自身抗体之间的反应，随后分离产生的免疫复合物和游离的聚乙二醇标记的甲状腺球蛋白。通过使用高度纯化的甲状腺球蛋白来获得标记物，确保了放射免疫分析的高特异性。结果表明该方法可用于自身免疫性甲状腺疾病的诊断。
Zotero 插件