Kargar M, Mojaver S, Namavari M, Sayari M, Rahimian A
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Trop Biomed. 2013 Jun;30(2):349-54.
There are some limiting aspects of scaling up the Neospora caninum tachyzoites in continuous cell lines, particularly as related to the absence of surface attachment. In this study, suspension cell culture of Theileria annulata-infected lymphoblastoid (TIL) was used as a host cell for the continous production of N. caninum tachyzoites. The numbers of free tachyzoites in the medium supernatant were showed regularly increased up to the day 6 post-cultivation. Transmission electron microscopy demonstrated that N. caninum tachyzoites invaded the TIL cells and multiplied intracellularly. This showed that the tachyzoites were successfully proliferated in TIL cells and were released in complete Dulbecco's modified Eagle's medium. This is a successful report of in vitro cultivation of N. caninum tachyzoites achieved by using suspension host cell culture.
在连续细胞系中扩大犬新孢子虫速殖子的培养存在一些限制因素，特别是与缺乏表面附着有关。在本研究中，用环形泰勒虫感染的淋巴母细胞样细胞（TIL）的悬浮细胞培养作为宿主细胞，用于连续生产犬新孢子虫速殖子。培养至第6天，培养基上清液中游离速殖子的数量呈规律性增加。透射电子显微镜显示犬新孢子虫速殖子侵入TIL细胞并在细胞内增殖。这表明速殖子在TIL细胞中成功增殖，并在完全的杜尔贝科改良伊格尔培养基中释放。这是通过使用悬浮宿主细胞培养实现犬新孢子虫速殖子体外培养的成功报告。
