Department of Cytology, Institute for Biological Research Sinisa Stankovic, University of Belgrade, Belgrade, Serbia.
J Physiol Pharmacol. 2013 Jun;64(3):361-8.
This study aimed to examine the effects of genistein on the structural and functional changes in parathyroid glands (PTG) and sodium phosphate cotransporter 2a (NaPi 2a) in orchidectomized rats. Sixteen-month-old Wistar rats were divided into sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30 mg/kg/day) was administered subcutaneously for 3 weeks, while the controls received vehicle alone. PTG was analyzed histomorphometrically, while the expressions of NaPi 2a mRNA/protein levels from kidneys were determined by real time PCR and Western blots. Serum and urine parameters were determined biochemically. The PTG volume in Orx rats was increased by 30% (p<0.05), compared to the SO group. Orx+G treatment increased the PTG volume by 35% and 75% (p<0.05) respectively, comparing to Orx and SO animals. Orchidectomy led to increment of serum PTH by 27% (p<0.05) compared to the SO group, Orx+G decreased it by 18% (p<0.05) comparing to Orx animals. NaPi 2a expression in Orx animals was reduced in regards to its abundance in SO animals, although it was increased in Orx+G group compared to the Orx. Phosphorus urine content of Orx animals was raised by 12% (p<0.05) compared to that for the SO group, while Orx+G induced a 17% reduction (p<0.05) in regards to Orx animals. Our study shows that Orx increases PTG volume and serum PTH level, while protein expression of NaPi 2a is reduced. Application of genistein attenuates the orchidectomy-induced changes in serum PTH level, stimulates the expression of NaPi 2a and reduces urinary Pi excretion, implying potential beneficial effects on andropausal symptoms.
本研究旨在探讨染料木黄酮对去势大鼠甲状旁腺（PTG）结构和功能变化以及钠磷共转运蛋白 2a（NaPi 2a）的影响。将 16 个月大的 Wistar 大鼠分为假手术（SO）、去势（Orx）和去势+染料木黄酮（Orx+G）组。染料木黄酮（30mg/kg/天）经皮给药 3 周，而对照组给予单独载体。通过实时 PCR 和 Western blot 法测定肾脏中 NaPi 2a mRNA/蛋白水平的表达，对 PTG 进行组织形态计量学分析。通过生化方法测定血清和尿液参数。与 SO 组相比，Orx 大鼠的 PTG 体积增加了 30%（p<0.05）。与 Orx 和 SO 动物相比，Orx+G 治疗分别使 PTG 体积增加了 35%和 75%（p<0.05）。与 SO 组相比，去势导致血清 PTH 增加 27%（p<0.05），而 Orx+G 组降低了 18%（p<0.05）。与 SO 动物相比，Orx 动物的 NaPi 2a 表达减少，但在 Orx+G 组中增加。与 SO 组相比，Orx 动物的尿磷含量增加了 12%（p<0.05），而 Orx+G 组则降低了 17%（p<0.05）。我们的研究表明，去势增加了 PTG 体积和血清 PTH 水平，而 NaPi 2a 的蛋白表达减少。染料木黄酮的应用减轻了去势引起的血清 PTH 水平变化，刺激了 NaPi 2a 的表达并减少了尿磷排泄，这表明其对更年期症状可能具有潜在的有益作用。
