先天性青光眼大鼠模型中的葡萄膜巩膜旁路功能障碍。

Dysfunctional uveoscleral pathway in a rat model of congenital glaucoma.

机构信息

Department of Ophthalmology, University Hospitals of Geneva HUG, Geneva, Switzerland.

出版信息

J Physiol Pharmacol. 2013 Jun;64(3):393-7.

Abstract

UNLABELLED

The purpose of the study was to investigate the presence of the uveoscleral pathway in the normotensive rat (NR) and in a rat model of congenital glaucoma (CGR). We injected the fluorescent tracer 70-kDa dextran rhodamine B in the anterior chamber of four NRs and four CGRs. At 10 and 60 minutes after injection, rats were euthanized by CO₂ inhalation and eyes were enucleated. Cryosections were prepared and analyzed using fluorescent microscopy. Hematoxylin-eosin staining and electron microscopy of the anterior chamber angle (ACA) were performed. At 10 minutes after injection, fluorescent tracer was detected in the iris root and ciliary processes of NRs and CGRs. At 60 minutes, NRs showed prominent signal in the suprachoroidal, whereas, in the CGRs, tracer was barely detectable. Histology of the anterior chamber revealed the presence of an open ACA and electron microscopy confirmed the normal structure of the ciliary body in CGRs.

CONCLUSIONS

Our results document the presence of an uveoscleral pathway in the normotensive rat. The rat model of congenital glaucoma shows severe impairment of the uveoscleral pathway, suggesting that alterations of the uveoscleral outflow might play a role in the pathogenesis of CG.

摘要

目的

本研究旨在探讨正常眼压大鼠（NR）和先天性青光眼（CGR）大鼠模型中葡萄膜巩膜途径的存在情况。我们将荧光示踪剂 70kDa 右旋糖酐 rhodamine B 注入 4 只 NR 和 4 只 CGR 的前房。注射后 10 和 60 分钟，用 CO₂吸入处死大鼠并眼球摘出。制备冷冻切片并用荧光显微镜进行分析。对前房角（ACA）进行苏木精-伊红染色和电子显微镜检查。注射后 10 分钟，NR 和 CGR 的虹膜根部和睫状突均检测到荧光示踪剂。60 分钟时，NR 显示出明显的脉络膜上腔信号，而 CGR 中的示踪剂几乎无法检测到。前房组织学显示 ACA 开放，电子显微镜证实 CGR 睫状体的正常结构。