Saghiri Mohammad Ali, Delvarani Abbas, Mehrvarzfar Payman, Nikoo Mohsen, Lotfi Mehrdad, Karamifar Kasra, Asgar Kamal, Dadvand Sahar
Department of Dental Material, Islamic Azad University (Dental Branch), Tehran, Iran.
Saudi Dent J. 2011 Jul;23(3):149-52. doi: 10.1016/j.sdentj.2011.03.002. Epub 2011 Mar 9.
Cytotoxicity of root canal irrigants is important due to their close contact with host tissues. This study was to assess the possible impact of pH on cytotoxic effects of MTAD, 17% EDTA, and 2.6% NaOCl on the human gingival fibroblasts using MTT assay.
Human gingival fibroblasts were exposed to the irrigants and their viability was assessed after 1, 6, and 12 h. The pH of the medium was measured in each interval. Light absorption values were measured for each culture medium using Elisa Reader device.
NaOCl had significantly less cytotoxicity than EDTA and MTAD. Also irrigants cytotoxicity decreased in 12, 1, and 6 h, respectively.
It seems that variation of the pH resulted in variation in the cytotoxicity of solutions; i.e., it follows the pattern of the pH variation.
由于根管冲洗剂与宿主组织密切接触，其细胞毒性很重要。本研究旨在使用MTT法评估pH对MTAD、17%乙二胺四乙酸（EDTA）和2.6%次氯酸钠（NaOCl）对人牙龈成纤维细胞细胞毒性作用的可能影响。
将人牙龈成纤维细胞暴露于冲洗剂中，并在1、6和12小时后评估其活力。在每个时间段测量培养基的pH值。使用酶标仪测量每种培养基的吸光值。
NaOCl的细胞毒性明显低于EDTA和MTAD。冲洗剂的细胞毒性分别在12、1和6小时降低。
似乎pH的变化导致了溶液细胞毒性的变化；即，它遵循pH变化的模式。
