孟鲁司特钠对哮喘患儿全血和分离的人多形核中性粒细胞（PMN）自由基生成的影响。

Effects of Montelukast on free radical production in whole blood and isolated human polymorphonuclear neutrophils (PMNs) in asthmatic children.

机构信息

Department of Paediatrics, College of Medicine, King Saud University, Riyadh, Saudi Arabia.

出版信息

Saudi Pharm J. 2011 Oct;19(4):215-20. doi: 10.1016/j.jsps.2011.06.002. Epub 2011 Jun 25.

DOI:10.1016/j.jsps.2011.06.002

PMID:23960762

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3745193/

Abstract

Montelukast is a highly selective leukotriene-receptor antagonist (LTRA). It is widely used in the treatment of bronchial asthma, primarily as an adjunct to corticosteroids. Reactive oxygen species (ROSs) play an important role in the pathogenesis of asthma and oxidative stress contributing to the initiation and worsening of inflammatory respiratory disorders, such as asthma. Antioxidant drugs may have a role in minimizing or preventing damage in asthmatic children. The aim of this study was to assess the antioxidant effect of montelukast on the production of free radicals in the whole blood and polymorphonuclear neutrophils (PMNs) in asthmatic children. A group of 48 (38 males and 10 females), apparently healthy asthmatic children were recruited with ages ranging between 6 and 14 years. In asthmatic children, base line (premedication) and post medication free radicals activity in the whole blood and polymorphonuclear neutrophils (PMNs) was determined by measuring chemiluminescence (CL) response through chemiluminescence luminometer. Free radical productions were significantly decreased in the whole blood, when stimulated with Phorbol Myristate Acetate (p < 0.04) and Opsonised Zymosan (p < 0.05). The free radicals were also significantly decreased in isolated polymorphonuclear neutrophils (PMNs) when stimulated with Opsonised Zymosan (p < 0.05) after the post medication treatment of montelukast in asthmatic children. Montelukast decreased the reactive oxygen species production, both in the whole blood as well as isolated PMNs in asthmatic children.

摘要

孟鲁司特是一种高度选择性的白三烯受体拮抗剂（LTRA）。它被广泛用于治疗支气管哮喘，主要作为皮质类固醇的辅助药物。活性氧（ROS）在哮喘发病机制中起着重要作用，氧化应激导致炎症性呼吸道疾病（如哮喘）的发生和恶化。抗氧化药物可能在减轻或预防哮喘儿童的损伤方面发挥作用。本研究旨在评估孟鲁司特对哮喘儿童全血和多形核粒细胞（PMN）中自由基产生的抗氧化作用。招募了一组 48 名（38 名男性和 10 名女性）年龄在 6 至 14 岁之间的明显健康的哮喘儿童。在哮喘儿童中，通过化学发光光度计测量化学发光（CL）反应，确定全血和多形核粒细胞（PMN）中基线（预用药）和用药后自由基活性。当用佛波醇肉豆蔻酸酯（p<0.04）和调理酵母聚糖刺激时，全血中的自由基产生显著降低（p<0.04）。在用孟鲁司特治疗哮喘儿童后，当用调理酵母聚糖刺激时，分离的多形核粒细胞（PMN）中的自由基也显著减少（p<0.05）。孟鲁司特降低了哮喘儿童全血和分离的 PMN 中活性氧的产生。