King Fahd Hospital, Gizan, Saudi Arabia.
Saudi J Biol Sci. 2011 Apr;18(2):213-8. doi: 10.1016/j.sjbs.2011.01.008. Epub 2011 Feb 3.
Cell culture systems have been established, where a hepatitis C virus (HCV) subgenomic replicon was efficiently replicated and maintained for a long period. It is known that HCV contains proteins which interact with host cell proteins. To see whether a HCV RNA replicon can interact in the same way with host cell proteins, HCV RNA replicon was transfected in Huh7 cells. In most infected cells, HCV replicon is present in the cytoplasm; however, in a minority of HCV-infected cells, both the cytoplasm and the nucleus or the nucleus on its own is positive for NS3. The presence of NS3 in the nuclei of Huh7 cells indicates that the protein may play a role other than in virus replication, such as in persistence of HCV infection.
已建立细胞培养系统，其中丙型肝炎病毒 (HCV) 亚基因组复制子可高效复制并长时间维持。已知 HCV 包含与宿主细胞蛋白相互作用的蛋白。为了观察 HCV RNA 复制子是否以相同方式与宿主细胞蛋白相互作用，将 HCV RNA 复制子转染入 Huh7 细胞。在大多数受感染的细胞中，HCV 复制子存在于细胞质中；然而，在少数 HCV 感染的细胞中，细胞质和细胞核或细胞核本身均为 NS3 阳性。NS3 存在于 Huh7 细胞的细胞核中表明该蛋白可能除了在病毒复制中发挥作用外，还可能在 HCV 感染的持续存在中发挥作用。
