Department of Internal Medicine, Section Nephrology and Transplantation, Erasmus Medical Center, Rotterdam, the Netherlands.
Clin Exp Immunol. 2013 Dec;174(3):424-32. doi: 10.1111/cei.12188.
Cytomegalovirus (CMV) infection has been implicated in accelerated T cell ageing. End-stage renal disease (ESRD) patients have a severely immunologically aged T cell compartment but also a high prevalence of CMV infection. We investigated whether CMV infection contributes to T cell ageing in ESRD patients. We determined the thymic output by the T cell receptor excision circle (TREC) content and percentage of CD31+ naïve T cells. The proliferative history of the T cell compartment by determination of the relative telomere length (RTL) and the T cell differentiation status was determined by immunophenotyping. It appeared that CMV infection did not affect thymic output but reduced RTL of CD8+ T cells in ESRD patients. Moreover, increased T cell differentiation was observed with higher percentages of CD57+ and CD28null CD4+ and CD8+ memory T cells. These CD28null T cells had significantly shorter telomeres compared to CD28+ T cells. Therefore we concluded that CMV infection does not affect the decreased thymic output but increases T cell differentiation as observed in ESRD-related premature T cell ageing.
巨细胞病毒 (CMV) 感染与 T 细胞加速衰老有关。终末期肾病 (ESRD) 患者的 T 细胞免疫功能严重老化，但 CMV 感染也很普遍。我们研究了 CMV 感染是否会导致 ESRD 患者的 T 细胞衰老。我们通过检测 T 细胞受体切除环 (TREC) 含量和 CD31+幼稚 T 细胞的百分比来确定胸腺输出。通过相对端粒长度 (RTL) 和 T 细胞分化状态的测定来确定 T 细胞的增殖历史。结果表明，CMV 感染并未影响 ESRD 患者的胸腺输出，但降低了 CD8+T 细胞的 RTL。此外，观察到 CD57+和 CD28null CD4+和 CD8+记忆 T 细胞的百分比增加，表明 T 细胞分化增加。这些 CD28null T 细胞的端粒明显短于 CD28+T 细胞。因此，我们得出结论，CMV 感染不会影响 ESRD 相关的 T 细胞过早衰老中观察到的减少的胸腺输出，但会增加 T 细胞分化。
