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没食子酸表没食子儿茶素酯减轻 L-DOPA 诱导的大鼠 PC12 细胞凋亡。

Epigallocatechin gallate attenuates L-DOPA-induced apoptosis in rat PC12 cells.

机构信息

Department of Food and Nutrition, College of Natural Science, Chosun University, 301, Pilmun-daero, Dong-gu, Gwangju 501-759, Korea.

出版信息

Nutr Res Pract. 2013 Aug;7(4):249-55. doi: 10.4162/nrp.2013.7.4.249. Epub 2013 Aug 7.

DOI:10.4162/nrp.2013.7.4.249
PMID:23964310
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3746157/
Abstract

In this study, the protective effects of EGCG on L-3,4-dihydroxyphenylalanine (L-DOPA)-induced oxidative cell death in catecholaminergic PC12 cells, the in vitro model of Parkinson's disease, were investigated. Treatment with L-DOPA at concentrations higher than 150 µM caused cytotoxicity in PC12 cells, as determined using the 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry detection. The apoptotic ratio was similar in cells treated with 100 µM EGCG plus 150 µM L-DOPA (5.02%) and the control (0.96%) (P > 0.05), and was lower than that of cells treated with L-DOPA only (32.24%, P < 0.05). The generation level of ROS (% of control) in cells treated with EGCG plus L-DOPA was lower than that in cells treated with L-DOPA only (123.90% vs 272.32%, P < 0.05). The optical density in production of TBARS in cells treated with L-DOPA only was higher than that in the control (0.27 ± 0.05 vs 0.08 ± 0.04, P < 0.05), and in cells treated with EGCG only (0.14 ± 0.02, P < 0.05), and EGCG plus L-DOPA (0.13 ± 0.02, P < 0.05). The intracellular level of GSH in cells treated with EGCG plus L-DOPA was higher than that in cells treated with L-DOPA only (233.25 ± 16.44 vs 119.23 ± 10.25, P < 0.05). These results suggest that EGCG protects against L-DOPA-induced oxidative apoptosis in PC12 cells, and might be a potent neuroprotective agent.

摘要

在这项研究中，我们研究了 EGCG 对 L-3,4-二羟基苯丙氨酸（L-DOPA）诱导的儿茶酚胺能 PC12 细胞（帕金森病的体外模型）氧化细胞死亡的保护作用。用浓度高于 150µM 的 L-DOPA 处理 PC12 细胞会导致细胞毒性，这可以通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定和流式细胞术检测来确定。用 100µM EGCG 和 150µM L-DOPA 处理的细胞的凋亡比例与对照（0.96%）相似（5.02%）（P>0.05），低于单独用 L-DOPA 处理的细胞（32.24%，P<0.05）。用 EGCG 和 L-DOPA 处理的细胞的 ROS 生成水平（对照的%）低于单独用 L-DOPA 处理的细胞（123.90%对 272.32%，P<0.05）。单独用 L-DOPA 处理的细胞中 TBARS 生成的光密度高于对照（0.27±0.05 对 0.08±0.04，P<0.05）和单独用 EGCG 处理的细胞（0.14±0.02，P<0.05），以及 EGCG 和 L-DOPA 处理的细胞（0.13±0.02，P<0.05）。用 EGCG 和 L-DOPA 处理的细胞内 GSH 水平高于单独用 L-DOPA 处理的细胞（233.25±16.44 对 119.23±10.25，P<0.05）。这些结果表明，EGCG 可防止 L-DOPA 诱导的 PC12 细胞氧化凋亡，可能是一种有效的神经保护剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b28/3746157/756a4c9c69c9/nrp-7-249-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b28/3746157/a4c65f773b60/nrp-7-249-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b28/3746157/da160dabbfe5/nrp-7-249-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b28/3746157/756a4c9c69c9/nrp-7-249-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b28/3746157/a4c65f773b60/nrp-7-249-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b28/3746157/da160dabbfe5/nrp-7-249-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b28/3746157/756a4c9c69c9/nrp-7-249-g007.jpg

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