[Phenothiazine derivatives as enhancers of peroxidase-dependent chemiluminescence].

Some N-alkyl phenothiazines with different ionic groups were studied as enhancers of chemiluminescence catalyzed by soybean peroxidase. Phenothiazines carrying positive charged groups had no an enhancement ability, whereas phenothiazine derivatives with negative charged groups increased significantly an intensity of chemiluminescence. Correlation between the enhancement activity ofphenothiazines and their ability to enzymatically oxidize by hydrogen peroxide was found. The discovery of new enhancers open good perspectives for an improvement of sensitivity of analytes determination by chemiluminescent enzyme immunoassay.