Chenu C, Kurihara N, Mundy G R, Roodman G D
Research Service, VA Medical Center, San Antonio, TX.
J Bone Miner Res. 1990 Jul;5(7):677-81. doi: 10.1002/jbmr.5650050703.
Prostaglandins are important local regulators of bone cell function and have been shown to have multiple effects on osteoclasts. Using a human bone marrow culture system in which multinucleated cells with osteoclast characteristics form, we have recently shown that TGF-beta is a potent inhibitor of osteoclastlike cell formation and appears to act at several stages of their development. Because it has been suggested that the effects of TGF-beta are mediated via a prostaglandin-dependent mechanism, we determined the effects of prostaglandin E2 (PGE2) on total and osteoclastlike cell formation (detected by reactivity with the 23c6 monoclonal antibody, which identifies osteoclasts) in human marrow cultures and tested whether prostaglandin synthesis was responsible for the inhibitory effects of TGF-beta on multinucleated cell formation. These studies show that PGE2 is a potent inhibitor of both 23c6-positive and negative multinucleate cell formation in human marrow cultures and that the effects of TGF-beta on multinucleated cell formation are not mediated by PGE2.
前列腺素是骨细胞功能重要的局部调节因子，已被证明对破骨细胞有多种作用。利用一种能形成具有破骨细胞特征的多核细胞的人骨髓培养系统，我们最近发现转化生长因子-β（TGF-β）是破骨细胞样细胞形成的有效抑制剂，并且似乎在其发育的几个阶段发挥作用。由于有人提出TGF-β的作用是通过一种前列腺素依赖性机制介导的，我们测定了前列腺素E2（PGE2）对人骨髓培养物中总细胞形成和破骨细胞样细胞形成（通过与识别破骨细胞的23c6单克隆抗体反应来检测）的影响，并测试了前列腺素合成是否是TGF-β对多核细胞形成抑制作用的原因。这些研究表明，PGE2是人类骨髓培养物中23c6阳性和阴性多核细胞形成的有效抑制剂，且TGF-β对多核细胞形成的作用不是由PGE2介导的。
