利用炭疽致死因子修饰的纳米颗粒从血清、唾液和泪液中高通量检测人中性粒细胞肽。
High throughput detection of human neutrophil peptides from serum, saliva, and tear by anthrax lethal factor-modified nanoparticles.
机构信息
CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology , 11 Beiyitiao, Zhongguancun, Beijing 100190, China.
出版信息
ACS Appl Mater Interfaces. 2013 Sep 11;5(17):8267-72. doi: 10.1021/am4021523. Epub 2013 Aug 28.
PMID:23965149
Abstract
Human α defensins human neutrophil peptide 1-3 (HNP 1-3) are potential prognostic cancer biomarkers. Metalloprotein anthrax lethal factor (ALF) binds to HNP 1-3 in a Zn2+-dependent manner. We conjugated ALF to the surface of magnetic nanoparticles (MNP) to magnetically isolate the HNPs, and used Zn2+ to control the capture and release HNPs.
摘要
人α防御素人中性粒细胞肽 1-3（HNP 1-3）是潜在的预后癌症生物标志物。金属蛋白酶炭疽致死因子（ALF）以 Zn2+依赖性方式与 HNP 1-3 结合。我们将 ALF 缀合到磁性纳米颗粒（MNP）的表面上，以通过磁场分离 HNP，并使用 Zn2+控制 HNP 的捕获和释放。
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