Department of Respiratory Medical Oncology, the Tumor Hospital of Harbin Medical University, Harbin, China.
Department of Pathology, the Tumor Hospital of Harbin Medical University, Harbin, China.
Histol Histopathol. 2014 Feb;29(2):273-8. doi: 10.14670/HH-29.273. Epub 2013 Aug 16.
Studies have confirmed that protein overexpression or mutations of KIT are involved in growth and development of a variety of cancers. however, little is known about data of gene mutation and protein expression in small cell lung cancer (SCLC) patients from northeast China.The aim of study is to investigate gene mutation and protein expression in such patients with small cell lung cancer (SCLC) and analyse their clinical significance.The expression of c-Kit protein was analyzed by immunohistochemistry in 77 SCLC samples and 22 normal lung samples. KIT mutations were screened in exons 9, 11, 13, 14, 17 and 18 by DNA direct sequencing.The study showed that positive staining for c-Kit was observed in 28 of 77 SCLC patients . There was no correlations between expression of c-Kit and sex, ages, smoking status, stage. only 1 case was found to have known T801I mutation in exon 17. The median survival (13.9 months) of cases with c-Kit-positive was shorter than that (19.9 months) of cases with c-Kit-negative. The finding revealed that stages was identified as an independent predictive factor for SCLC patients.Our finding reveals that somatic mutation of KIT is rare in SCLC patients from the northeast China and there is no enough evidence comfirming KIT inhibitors for treatment in SCLC.
研究证实，KIT 的蛋白过表达或突变与多种癌症的生长和发展有关。然而，对于来自中国东北地区的小细胞肺癌（SCLC）患者的基因突变和蛋白表达数据知之甚少。本研究旨在探讨小细胞肺癌（SCLC）患者的基因突变和蛋白表达情况，并分析其临床意义。采用免疫组织化学方法检测 77 例 SCLC 组织和 22 例正常肺组织中 c-Kit 蛋白的表达情况，采用直接 DNA 测序法检测 KIT 基因外显子 9、11、13、14、17 和 18 的突变情况。研究结果显示，77 例 SCLC 患者中有 28 例 c-Kit 阳性染色。c-Kit 表达与性别、年龄、吸烟状态、分期均无相关性，仅 1 例患者在第 17 外显子发现已知的 T801I 突变。c-Kit 阳性患者的中位生存时间（13.9 个月）短于 c-Kit 阴性患者（19.9 个月）。研究表明，分期是 SCLC 患者的独立预后预测因素。本研究揭示了中国东北地区 SCLC 患者中 KIT 体细胞突变罕见，且无足够证据证实 KIT 抑制剂可用于 SCLC 的治疗。
