Institute of Phytopathology and Applied Zoology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
Int J Mol Sci. 2013 Aug 20;14(8):17122-46. doi: 10.3390/ijms140817122.
Bacterial quorum sensing molecules not only grant the communication within bacterial communities, but also influence eukaryotic hosts. N-acyl-homoserine lactones (AHLs) produced by pathogenic or beneficial bacteria were shown to induce diverse reactions in animals and plants. In plants, the reaction to AHLs depends on the length of the lipid side chain. Here we investigated the impact of two bacteria on Arabidopsis thaliana, which usually enter a close symbiosis with plants from the Fabaceae (legumes) family and produce a long-chain AHL (Sinorhizobium meliloti) or a short-chain AHL (Rhizobium etli). We demonstrate that, similarly to the reaction to pure AHL molecules, the impact, which the inoculation with rhizosphere bacteria has on plants, depends on the type of the produced AHL. The inoculation with oxo-C14-HSL-producing S. meliloti strains enhanced plant resistance towards pathogenic bacteria, whereas the inoculation with an AttM lactonase-expressing S. meliloti strain did not. Inoculation with the oxo-C8-HSL-producing R. etli had no impact on the resistance, which is in agreement with our previous hypothesis. In addition, plants seem to influence the availability of AHLs in the rhizosphere. Taken together, this report provides new insights in the role of N-acyl-homoserine lactones in the inter-kingdom communication at the root surface.
细菌群体感应分子不仅赋予了细菌群落内部的通讯能力，还影响了真核宿主。已证实，由病原菌或有益菌产生的 N-酰基高丝氨酸内酯（AHLs）会在动物和植物中引发多种反应。在植物中，对 AHLs 的反应取决于脂酰侧链的长度。在这里，我们研究了两种细菌对拟南芥的影响，这两种细菌通常与豆科（豆类）植物形成密切共生关系，并产生长链 AHL（根瘤菌）或短链 AHL（根瘤菌）。我们证明，类似于对纯 AHL 分子的反应，接种根际细菌对植物的影响取决于所产生 AHL 的类型。接种产生 oxo-C14-HSL 的 S. meliloti 菌株增强了植物对病原菌的抗性，而接种表达 AttM 内酯酶的 S. meliloti 菌株则没有。接种产生 oxo-C8-HSL 的 R. etli 对抗性没有影响，这与我们之前的假设一致。此外，植物似乎影响根际中 AHLs 的可用性。总之，本报告为 N-酰基高丝氨酸内酯在根表面的种间通讯中的作用提供了新的见解。
