一种新型的全血样本输送和检测方法：用于即时护理（POC）分子诊断的凝胶条 PCR。
A novel method for sample delivery and testing of whole blood: gel strip PCR for point of care (POC) molecular diagnostics.
机构信息
Department of Oncology, University of Alberta and Cross Cancer Institute, 11560 University Avenue, Edmonton, AB T6G 1Z2, Canada.
出版信息
Lab Chip. 2013 Oct 21;13(20):4011-4. doi: 10.1039/c3lc50755f. Epub 2013 Aug 22.
PMID:23966268
Abstract
Testing of whole blood in miniaturized PCR is compromised by the opaque nature of whole blood that leads to physical masking of a fluorescent signal. We demonstrate a method to perform real-time PCR with whole blood that avoids interference from the opacity of whole blood.
摘要
全血的微型 PCR 测试受到全血不透明性质的影响，导致荧光信号的物理屏蔽。我们展示了一种避免全血不透明度干扰的实时 PCR 方法。
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