通过不同的 5' 和 3' UTR 元件在神经元群体中进行神经粘蛋白/CPG15 mRNA 的轴突定位。
Axonal localization of neuritin/CPG15 mRNA in neuronal populations through distinct 5' and 3' UTR elements.
机构信息
Department of Biology, Drexel University, Philadelphia, PA 19104, USA.
出版信息
J Neurosci. 2013 Aug 21;33(34):13735-42. doi: 10.1523/JNEUROSCI.0962-13.2013.
Abstract
Many neuronal mRNAs are actively transported into distal axons. The 3' untranslated regions (UTRs) of axonal mRNAs often contain cues for their localization. The 3' UTR of neuritin mRNA was shown to be sufficient for localization into axons of hippocampal neurons. Here, we show that neuritin mRNA localizes into axons of rat sensory neurons, but this is predominantly driven by the 5' rather than 3' UTR. Neuritin mRNA shifts from cell body to axon predominantly after nerve crush injury, suggesting that it encodes a growth-associated protein. Consistent with this, overexpression of neuritin increases axon growth but only when its mRNA localizes into the axons.
摘要
许多神经元 mRNA 被主动运输到远端轴突中。轴突 mRNA 的 3'非翻译区 (UTR) 通常包含定位信号。神经黏蛋白 mRNA 的 3'UTR 足以使其定位于海马神经元的轴突中。在这里，我们表明神经黏蛋白 mRNA 定位于大鼠感觉神经元的轴突中，但主要是由 5'UTR 而不是 3'UTR 驱动的。神经黏蛋白 mRNA 在神经挤压损伤后主要从细胞体转移到轴突，表明它编码一种与生长相关的蛋白质。与之一致的是，神经黏蛋白的过表达增加了轴突的生长，但只有当它的 mRNA 定位于轴突中时才会发生这种情况。
相似文献
1
Axonal localization of neuritin/CPG15 mRNA in neuronal populations through distinct 5' and 3' UTR elements.通过不同的 5' 和 3' UTR 元件在神经元群体中进行神经粘蛋白/CPG15 mRNA 的轴突定位。
J Neurosci. 2013 Aug 21;33(34):13735-42. doi: 10.1523/JNEUROSCI.0962-13.2013.
2
Axonal amphoterin mRNA is regulated by translational control and enhances axon outgrowth.轴突双调蛋白信使核糖核酸受翻译控制调节，并增强轴突生长。
J Neurosci. 2015 Apr 8;35(14):5693-706. doi: 10.1523/JNEUROSCI.3397-14.2015.
3
Axonal localization of neuritin/CPG15 mRNA is limited by competition for HuD binding.神经黏附素/CPG15 mRNA 的轴突定位受 HuD 结合竞争限制。
J Cell Sci. 2017 Nov 1;130(21):3650-3662. doi: 10.1242/jcs.201244. Epub 2017 Sep 4.
4
A HuD-ZBP1 ribonucleoprotein complex localizes GAP-43 mRNA into axons through its 3' untranslated region AU-rich regulatory element.HuD-ZBP1 核糖核蛋白复合物通过其 3' 非翻译区富含 AU 的调控元件将 GAP-43 mRNA 定位到轴突中。
J Neurochem. 2013 Sep;126(6):792-804. doi: 10.1111/jnc.12266. Epub 2013 Apr 30.
5
Axonal Localization of transgene mRNA in mature PNS and CNS neurons.转基因 mRNA 在成熟 PNS 和 CNS 神经元中的轴突定位。
J Neurosci. 2011 Oct 12;31(41):14481-7. doi: 10.1523/JNEUROSCI.2950-11.2011.
6
Axonal tau mRNA localization coincides with tau protein in living neuronal cells and depends on axonal targeting signal.轴突tau信使核糖核酸的定位与活神经元细胞中的tau蛋白一致，且依赖于轴突靶向信号。
J Neurosci. 2001 Sep 1;21(17):6577-87. doi: 10.1523/JNEUROSCI.21-17-06577.2001.
7
Alternative 3' UTRs Modify the Localization, Regulatory Potential, Stability, and Plasticity of mRNAs in Neuronal Compartments.非经典 3'UTR 改变神经元区 mRNA 的定位、调控潜能、稳定性和可塑性。
Neuron. 2018 May 2;98(3):495-511.e6. doi: 10.1016/j.neuron.2018.03.030. Epub 2018 Apr 12.
8
Possible Cis-acting signal that could be involved in the localization of different mRNAs in neuronal axons.可能参与不同mRNA在神经元轴突中定位的顺式作用信号。
Theor Biol Med Model. 2005 Aug 24;2:33. doi: 10.1186/1742-4682-2-33.
9
Trypanosoma cruzi: modulation of HSP70 mRNA stability by untranslated regions during heat shock.克氏锥虫：热休克过程中通过非翻译区调节 HSP70 mRNA 的稳定性。
Exp Parasitol. 2010 Oct;126(2):245-53. doi: 10.1016/j.exppara.2010.05.009. Epub 2010 May 21.
10
Identification of proteins interacting with HSP70 mRNAs in Leishmania braziliensis.鉴定巴西利什曼原虫中与 HSP70 mRNA 相互作用的蛋白质。
J Proteomics. 2013 Dec 6;94:124-37. doi: 10.1016/j.jprot.2013.09.008. Epub 2013 Sep 20.
引用本文的文献
1
Regulation of Subcellular Protein Synthesis for Restoring Neural Connectivity.用于恢复神经连接的亚细胞蛋白质合成调控
Int J Mol Sci. 2025 Jul 28;26(15):7283. doi: 10.3390/ijms26157283.
2
Disruption of G3BP1 granules promotes mammalian CNS and PNS axon regeneration.G3BP1颗粒的破坏促进哺乳动物中枢神经系统和外周神经系统轴突再生。
Proc Natl Acad Sci U S A. 2025 Mar 4;122(9):e2411811122. doi: 10.1073/pnas.2411811122. Epub 2025 Feb 27.
3
KHSRP-mediated Decay of Axonally Localized Prenyl-Cdc42 mRNA Slows Nerve Regeneration.KHSRP介导的轴突定位的异戊二烯化Cdc42 mRNA的降解减缓神经再生。
bioRxiv. 2025 Feb 8:2025.02.06.636857. doi: 10.1101/2025.02.06.636857.
4
RNA and neuronal function: the importance of post-transcriptional regulation.RNA与神经元功能：转录后调控的重要性
Oxf Open Neurosci. 2022 Jul 7;1:kvac011. doi: 10.1093/oons/kvac011. eCollection 2022.
5
Presynaptic perspective: Axonal transport defects in neurodevelopmental disorders.突触前观点：神经发育障碍中的轴突运输缺陷。
J Cell Biol. 2024 Jun 3;223(6). doi: 10.1083/jcb.202401145. Epub 2024 Apr 3.
6
The dynamic world of RNA: beyond translation to subcellular localization and function.RNA的动态世界：从翻译到亚细胞定位及功能的拓展
Front Genet. 2024 Mar 12;15:1373899. doi: 10.3389/fgene.2024.1373899. eCollection 2024.
7
Control of Selective mRNA Translation in Neuronal Subcellular Compartments in Health and Disease.控制健康和疾病中神经元亚细胞区室中选择性 mRNA 翻译。
J Neurosci. 2023 Nov 1;43(44):7247-7263. doi: 10.1523/JNEUROSCI.2240-22.2023.
8
Untranslated regions of brain-derived neurotrophic factor mRNA control its translatability and subcellular localization.脑源性神经营养因子 mRNA 的未翻译区控制其翻译能力和亚细胞定位。
J Biol Chem. 2023 Feb;299(2):102897. doi: 10.1016/j.jbc.2023.102897. Epub 2023 Jan 11.
9
Intra-axonal translation of Khsrp mRNA slows axon regeneration by destabilizing localized mRNAs.Khsrp mRNA 的轴内翻译通过破坏局部 mRNA 来减缓轴突再生。
Nucleic Acids Res. 2022 Jun 10;50(10):5772-5792. doi: 10.1093/nar/gkac337.
10
Axonal mRNA localization and translation: local events with broad roles.轴突 mRNA 定位和翻译：具有广泛作用的局部事件。
Cell Mol Life Sci. 2021 Dec;78(23):7379-7395. doi: 10.1007/s00018-021-03995-4. Epub 2021 Oct 26.
本文引用的文献
1
A HuD-ZBP1 ribonucleoprotein complex localizes GAP-43 mRNA into axons through its 3' untranslated region AU-rich regulatory element.HuD-ZBP1 核糖核蛋白复合物通过其 3' 非翻译区富含 AU 的调控元件将 GAP-43 mRNA 定位到轴突中。
J Neurochem. 2013 Sep;126(6):792-804. doi: 10.1111/jnc.12266. Epub 2013 Apr 30.
2
Axonally synthesized β-actin and GAP-43 proteins support distinct modes of axonal growth.轴突合成的β-肌动蛋白和 GAP-43 蛋白支持不同的轴突生长模式。
J Neurosci. 2013 Feb 20;33(8):3311-22. doi: 10.1523/JNEUROSCI.1722-12.2013.
3
Axonal transport of neural membrane protein 35 mRNA increases axon growth.神经膜蛋白 35mRNA 的轴突运输促进轴突生长。
J Cell Sci. 2013 Jan 1;126(Pt 1):90-102. doi: 10.1242/jcs.107268. Epub 2012 Oct 24.
4
Subcellular knockout of importin β1 perturbs axonal retrograde signaling.细胞质内敲除 importin β1 会扰乱轴突逆行信号转导。
Neuron. 2012 Jul 26;75(2):294-305. doi: 10.1016/j.neuron.2012.05.033.
5
Lysophosphatidic acid differentially regulates axonal mRNA translation through 5'UTR elements.溶血磷脂酸通过 5'UTR 元件差异调控轴突 mRNA 翻译。
Mol Cell Neurosci. 2012 Jun;50(2):136-46. doi: 10.1016/j.mcn.2012.04.001. Epub 2012 Apr 10.
6
Axonal mRNA localization and local protein synthesis in nervous system assembly, maintenance and repair.轴突 mRNA 定位与局部蛋白质合成在神经系统组装、维持和修复中的作用。
Nat Rev Neurosci. 2012 Apr 13;13(5):308-24. doi: 10.1038/nrn3210.
7
CPG15 regulates synapse stability in the developing and adult brain.CPG15 调节发育中和成年大脑中的突触稳定性。
Genes Dev. 2011 Dec 15;25(24):2674-85. doi: 10.1101/gad.176172.111.
8
Axonal Localization of transgene mRNA in mature PNS and CNS neurons.转基因 mRNA 在成熟 PNS 和 CNS 神经元中的轴突定位。
J Neurosci. 2011 Oct 12;31(41):14481-7. doi: 10.1523/JNEUROSCI.2950-11.2011.
9
Limited availability of ZBP1 restricts axonal mRNA localization and nerve regeneration capacity.ZBP1 的有限可用性限制了轴突 mRNA 的定位和神经再生能力。
EMBO J. 2011 Sep 30;30(22):4665-77. doi: 10.1038/emboj.2011.347.
10
Interaction of survival of motor neuron (SMN) and HuD proteins with mRNA cpg15 rescues motor neuron axonal deficits.运动神经元存活（SMN）蛋白和 HuD 蛋白与 mRNA cpg15 的相互作用可挽救运动神经元轴突缺陷。
Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10337-42. doi: 10.1073/pnas.1104928108. Epub 2011 Jun 7.
Zotero 插件