Department of Proteomics, Helmholtz-Centre for Environmental Research, Leipzig, Germany.
J Investig Allergol Clin Immunol. 2013;23(3):159-67.
Globalization of the food industry has led to widespread exposure to new nondomestic fish species; therefore, identification of potential allergens is necessary in order to diagnose allergic reactions.
Contact with a patient who was allergic to Nile perch (Lates niloticus) prompted us to investigate the immunoglobulin (Ig) E-reactive proteins that could be allergens of this species.
2D gel electrophoresis was used to separate the muscle proteins of L niloticus and Gadus morhua. Immunoblotting was performed with sera from 12 patients with a history of immediate-type allergic reaction to fish and from atopic and nonatopic controls. IgE-reactive proteins were detected and identified using mass spectrometry.
The index patient had low levels of IgE binding to parvalbumins. However, 8 putative allergens other than parvalbumin from L niloticus and 5 from G morhua were identified. Further investigation revealed cross-sensitivity to enolase 3 from L niloticus in 7 of the 12 fish-allergic individuals (58%), whereas 11 of the 12 patients (92%) were sensitized to enolase 3 from G morhua. However, atopic control patients were also sensitized to enolase 3 from L niloticus and G morhua.
Identification of species-specific allergens and individual sensitization could help us to improve avoidance strategies.
食品行业的全球化导致人们广泛接触到新的非本地鱼类；因此，有必要识别潜在的过敏原，以便诊断过敏反应。
接触到一位对尼罗河鲈鱼（Lates niloticus）过敏的患者，促使我们研究可能是该物种过敏原的免疫球蛋白（Ig）E 反应蛋白。
使用 2D 凝胶电泳分离 L 尼罗罗非鱼和鳕鱼的肌肉蛋白。用 12 名有鱼类即时型过敏反应史的患者和特应性及非特应性对照者的血清进行免疫印迹。使用质谱法检测和鉴定 IgE 反应蛋白。
该索引患者的 IgE 与副肌球蛋白结合水平较低。然而，除了副肌球蛋白外，还从 L 尼罗罗非鱼和 G 鳕鱼中鉴定出 8 种潜在的过敏原。进一步的研究表明，在 12 名鱼类过敏者中，有 7 名（58%）对 L 尼罗罗非鱼的烯醇酶 3 存在交叉敏感性，而 12 名患者中的 11 名（92%）对 G 鳕鱼的烯醇酶 3 敏感。然而，特应性对照组患者也对 L 尼罗罗非鱼和 G 鳕鱼的烯醇酶 3 敏感。
鉴定出物种特异性过敏原和个体致敏性可以帮助我们改进回避策略。
