Gilson S D, Parker B B, Twedt D C
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
Am J Vet Res. 1990 Sep;51(9):1385-7.
To evaluate the sensitivity and specificity of 2 commercial test kits for detection of occult blood in canine feces, various volumes of blood were administered to 6 dogs via orogastric tube. Blood volumes tested were chosen on the basis of hemoglobin quantities of 5, 10, 20, 200, 350, and 500 mg of hemoglobin/kg of body weight. Fecal specimens were collected twice daily and analyzed separately by 2 observers for the presence of occult blood by use of modified guaiac and orthotolodine tablet tests, and for melena by visual inspection. Five dogs given blood at the rate of 500 mg of hemoglobin/kg and 1 dog given blood at the rate of 350 mg of hemoglobin/kg developed melena. Results of both occult blood tests were positive in 2 of 6 dogs given blood at the rate of 5 mg of hemoglobin/kg. Five of 6, and 4 of 6 dogs given blood at the rate of 10 mg hemoglobin/kg had positive test results by modified guaiac and orthotolodine methods, respectively. Results of both methods were positive in all dogs given blood at the rate of 20 mg of hemoglobin/kg. There was 86% agreement between the 2 observers' results for the modified guaiac method, and 78% agreement for the orthotolodine method. There was 77% agreement of results between the 2 test methods. Gastrointestinal transit time decreased with increasing volumes of blood. Occult blood testing was found to be useful for detection of blood in feces at volumes 20 to 50 times less than that required to cause melena.
为评估2种用于检测犬粪便潜血的商用检测试剂盒的敏感性和特异性，通过口胃管给6只犬给予不同体积的血液。所测试的血量是根据每千克体重5、10、20、200、350和500毫克血红蛋白的量来选择的。每天收集两次粪便标本，由2名观察者分别使用改良愈创木脂和邻联甲苯胺片剂试验分析潜血情况，并通过肉眼检查分析有无黑粪症。5只以每千克体重500毫克血红蛋白的速率输血的犬和1只以每千克体重350毫克血红蛋白的速率输血的犬出现了黑粪症。在以每千克体重5毫克血红蛋白的速率输血的6只犬中，有2只犬的两种潜血试验结果均为阳性。在以每千克体重10毫克血红蛋白的速率输血的6只犬中，分别有5只和4只犬通过改良愈创木脂法和邻联甲苯胺法得到阳性试验结果。在以每千克体重20毫克血红蛋白的速率输血的所有犬中，两种方法的结果均为阳性。两名观察者对改良愈创木脂法的结果一致性为86%，对邻联甲苯胺法的结果一致性为78%。两种检测方法的结果一致性为77%。胃肠通过时间随着输血量的增加而缩短。发现潜血检测对于检测粪便中血量比引起黑粪症所需血量少20至50倍的血液很有用。
