乙酰辅酶A羧化酶。辅基在酶聚合中的作用。
Acetyl-Coenzyme A carboxylase. Role of the prosthetic group in enzyme polymerization.
作者信息
Landman A D, Darkshinamurti K
出版信息
Biochem J. 1975 Mar;145(3):545-8. doi: 10.1042/bj1450545.
Abstract
Apo-(acetyl-CoA carboxylase) completely free from the holoenzyme was prepared from biotin-deficient rat adipose tissue by using affinity chromatography. The apoenzyme does not aggregate under conditions favouring the transition of the holoenzyme to the polymeric form. Such transition is possible after the conversion of the apoenzyme into the holoenzyme in vitro, thus demonstrating the requirement of the prosthetic biotinyl group for enzyme activation.
摘要
通过亲和层析法从生物素缺乏的大鼠脂肪组织中制备出完全不含全酶的脱辅基（乙酰辅酶A羧化酶）。在有利于全酶向聚合形式转变的条件下，脱辅基酶不会聚集。在体外将脱辅基酶转化为全酶后，这种转变是可能的，从而证明了辅基生物素基团对酶激活的必要性。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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2
The mechanism of tricarboxylic acid cycle regulation of fatty acid synthesis.三羧酸循环对脂肪酸合成的调控机制。
J Biol Chem. 1962 Jun;237:1787-92.
3
4
Studies on the mechnism of activation of acetyl coenzyme A carboxylase by citrate.关于柠檬酸激活乙酰辅酶A羧化酶机制的研究。
J Biol Chem. 1963 Feb;238:533-40.
5
Acetyl-CoA carboxylase from rat adipose tissue.来自大鼠脂肪组织的乙酰辅酶A羧化酶。
Biochim Biophys Acta. 1969 Mar 4;176(2):221-9. doi: 10.1016/0005-2760(69)90181-7.
6
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