肾上腺素与大鼠附睾脂肪组织中乙酰辅酶A羧化酶的调节。该酶的失活与磷酸化有关，去磷酸化后可逆转。
Adrenaline and the regulation of acetyl-coenzyme A carboxylase in rat epididymal adipose tissue. Inactivation of the enzyme is associated with phosphorylation and can be reversed on dephosphorylation.
作者信息
Brownsey R W, Hughes W A, Denton R M
出版信息
Biochem J. 1979 Oct 15;184(1):23-32. doi: 10.1042/bj1840023.
Abstract
- Exposure of rat epididymal fat-pads or isolated fat-cells to adrenaline results in a decrease in acetyl-CoA carboxylase activity measured both in initial extracts and in extracts incubated with potassium citrate; in addition the concentration of citrate required to give half-maximal activation may also be increased. 2. Incorporation of 32Pi into acetyl-CoA carboxylase within intact fat-cells was investigated and evidence is presented that adrenaline increases the extent of phosphorylation of the enzyme. 3. Dephosphorylation of 32P-labelled acetyl-CoA carboxylase was studied in cell extracts. The rate of release of 32P is increased by 5mM-MgCl2 plus 10--100 microM-Ca2+, whereas it is inhibited by the presence of bivalent metal ion chelators such as EDTA and citrate. 4. The effects of adrenaline on the kinetic properties of acetyl-CoA carboxylase disappear if pad or cell extracts are treated with Mg2+ and Ca2+ under conditions that also lead to dephosphorylation of the enzyme. 5. The results of this study represent convincing evidence that adrenaline inactivates acetyl-CoA carboxylase in adipose-tissue preparations by increasing the degree of phosphorylation of the enzyme.
摘要
- 将大鼠附睾脂肪垫或分离的脂肪细胞暴露于肾上腺素会导致在初始提取物以及与柠檬酸钾一起孵育的提取物中测得的乙酰辅酶A羧化酶活性降低；此外，产生半最大激活所需的柠檬酸盐浓度也可能增加。2. 研究了完整脂肪细胞内32P掺入乙酰辅酶A羧化酶的情况，并提供了证据表明肾上腺素会增加该酶的磷酸化程度。3. 在细胞提取物中研究了32P标记的乙酰辅酶A羧化酶的去磷酸化。5mM氯化镁加10 - 100微摩尔钙离子会增加32P的释放速率，而二价金属离子螯合剂如乙二胺四乙酸和柠檬酸盐的存在则会抑制其释放。4. 如果在导致该酶去磷酸化的条件下用镁离子和钙离子处理脂肪垫或细胞提取物，肾上腺素对乙酰辅酶A羧化酶动力学性质的影响就会消失。5. 本研究结果提供了令人信服的证据，即肾上腺素通过增加该酶的磷酸化程度使脂肪组织制剂中的乙酰辅酶A羧化酶失活。
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本文引用的文献
1
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
2
Differential effects of metabolites on the active and inactive forms of hepatic acetyl CoA carboxylase.
Arch Biochem Biophys. 1974 Oct;164(2):490-501. doi: 10.1016/0003-9861(74)90059-9.
3
Regulation of hepatic acetyl coenzyme A carboxylase by phosphorylation and dephosphorylation.
Arch Biochem Biophys. 1974 Oct;164(2):478-89. doi: 10.1016/0003-9861(74)90058-7.
4
Hormonal regulation of adipose-tissue acetyl-Coenzyme A carboxylase by changes in the polymeric state of the enzyme. The role of long-chain fatty acyl-Coenzyme A thioesters and citrate.
Biochem J. 1974 Aug;142(2):365-77. doi: 10.1042/bj1420365.
5
Acetyl coenzyme A carboxylase.
Curr Top Cell Regul. 1974;8(0):139-95.
6
Insulin and the regulation of adipose tissue acetyl-coenzyme A carboxylase.
Biochem J. 1973 Mar;132(3):509-17. doi: 10.1042/bj1320509.
7
Identification and subcellular distribution of adipocyte peptides and phosphopeptides.
J Biol Chem. 1976 Mar 10;251(5):1505-10.
8
Effect of glucagon on hepatic malonyl coenzyme A concentration and on lipid synthesis.
J Biol Chem. 1977 Jun 25;252(12):4421-4.
9
Exchangeable and total calcium pools in mitochondria of rat epididymal fat-pads and isolated fat-cells. Role in the regulation of pyruvate dehydrogenase activity.
Biochem J. 1976 Jan 15;154(1):209-23. doi: 10.1042/bj1540209.
10
Action of insulin and catecholamines on the phosphorylation of proteins associated with the cytosol, membranes, and "fat cake" of rat fat cells.
J Biol Chem. 1978 Mar 10;253(5):1700-9.
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