猪肩胛骨软骨无机焦磷酸酶的纯化及异质性
Purification and heterogeneity of inorganic pyrophosphatase of pig scapula cartilage.
作者信息
Felix R, Fleisch H
出版信息
Biochem J. 1975 Apr;147(1):103-9. doi: 10.1042/bj1470103.
Abstract
Inorganic pyrophosphatase (pyrophosphate phosphohydrolase; EC 3.6.1.1) was purified from pig scapula cartilage by fractionation with N-cetylpyridinium chloride and (NH4)2SO4, followed by ion-exchange and gel-filtration chromatography. Enzyme preparations of high purity were obtained, with specific activities (100-400 units/mg) higher than those previously described for mammalian pyrophosphatases. The enzyme activity could be separated into several subfractions on ion-exchange columns.
摘要
无机焦磷酸酶（焦磷酸磷酸水解酶；EC 3.6.1.1）通过用十六烷基氯化吡啶和硫酸铵分级分离，随后进行离子交换和凝胶过滤色谱法，从猪肩胛骨软骨中纯化得到。获得了高纯度的酶制剂，其比活性（100 - 400单位/毫克）高于先前报道的哺乳动物焦磷酸酶。在离子交换柱上，酶活性可被分离成几个亚级分。
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