Mahuad Sabina L, Pratta Guillermo R, Rodriguez Gustavo R, Zorzoli Roxana, Picardi Liliana A
Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET), and Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, CC 14 S2125ZAA, Zavalla (Santa Fe), Argentina.
J Genet. 2013;92(2):195-203. doi: 10.1007/s12041-013-0245-z.
Five recombinant inbred lines obtained from the F2 generation of an interspecific cross between cultivar, Caimanta (Cai, Solanum lycopersicum) and wild accession, LA722 (P, S. pimpinellifolium) were crossed to obtain the second cycle hybrids (SCH). Eleven fruit quality traits were assessed in evaluating phenotypic variability among genotypes P, Cai, F1 (Cai x P), five RILs, and 10 SCH. One of the five recombinant inbred lines and three SCH had higher values than P, as the best genotype for shelf life. Sequence-related amplified polymorphism was used as the molecular method for detecting polymorphism among these 18 genotypes. The percentage of polymorphism in RILs and SCH was 61% and 66% respectively. Moreover, some bands detected in P were present in SCH. Several multivariate analyses were performed to find agreement between the phenotypic variability observed for fruit quality traits and the polymorphism obtained from sequence-related amplified polymorphism markers. A general Procrustes analysis estimated that there was a consensus proportion of 75% between phenotypic and molecular data. There was considerable preservation of some bands from the wild genotype, which could increase the variability in fruit quality traits in populations where the genetic diversity is limited.
从栽培品种Caimanta（Cai，番茄）与野生种质LA722（P，醋栗番茄）种间杂交的F2代获得的5个重组自交系进行杂交，以获得第二代杂种（SCH）。对基因型P、Cai、F1（Cai×P）、5个重组自交系和10个SCH的11个果实品质性状进行评估，以评价基因型间的表型变异性。其中一个重组自交系和三个SCH的货架期值高于P，P是货架期最佳基因型。采用序列相关扩增多态性作为检测这18个基因型多态性的分子方法。重组自交系和SCH的多态性百分比分别为61%和66%。此外，在P中检测到的一些条带也存在于SCH中。进行了多项多变量分析，以找出果实品质性状观察到的表型变异性与序列相关扩增多态性标记获得的多态性之间的一致性。一般Procrustes分析估计，表型数据和分子数据之间的一致比例为75%。野生基因型的一些条带得到了相当程度的保留，这可能会增加遗传多样性有限的群体中果实品质性状的变异性。
