Ogier-Denis E, Bauvy C, Aubery M, Codogno P, Sapin C, Darmoul D, Zweibaum A, Trugnan G
UFR Biomédicale des Saint-Péres, INSERM U180, Unité de Recherches sur la Biologie et Pathologie Moléculaires des Glycoprotéines, Paris, France.
Reprod Nutr Dev. 1990;30(3):325-30. doi: 10.1051/rnd:19900305.
The human colon cancer cell line HT-29 remains totally undifferentiated when glucose is present in the culture medium (HT-29 Glc+), while the same cells may undergo typical enterocytic differentiation after reaching confluence when grown in glucose-deprived medium (HT-29 Glc-). Recently, we demonstrated a deficiency in the overall N-glycan processing in confluent undifferentiated cells, whereas differentiated cells follow a classical pattern of N-glycosylation. The main changes in N-glycosylation observed in confluent undifferentiated cells may be summarised as follows: 1) the conversion of high mannose into complex glycopeptides is greatly decreased; 2) this decreased conversion could be a consequence of an accumulation of Man9-8-GlcNAc2-Asn high mannose species. Whether these changes in N-glycan processing appear progressively during cell culture or are already present from the beginning of the culture was investigated in this study by comparing the actual status of N-glycan processing in exponentially growing HT-29 Glc- and HT-29 Glc+ cells. Under these conditions, HT-29 Glc- cells do not exhibit any characteristics of differentiation. The conversion of high mannose into complex glycoproteins is severely reduced in HT-29 Glc+ cells, regardless of the growth phase studied. In contrast, HT-29 Glc- cells display a normal pattern of N-glycan processing in both growth phases. We therefore conclude that N-glycan processing may be used as an early biochemical marker of the enterocytic differentiation process of HT-29 cells.
人结肠癌细胞系HT - 29在培养基中存在葡萄糖时（HT - 29 Glc +）保持完全未分化状态，而同样的细胞在葡萄糖缺乏的培养基中生长达到汇合后可能会经历典型的肠上皮细胞分化（HT - 29 Glc -）。最近，我们证明汇合的未分化细胞在整体N -聚糖加工方面存在缺陷，而分化细胞遵循经典的N -糖基化模式。在汇合的未分化细胞中观察到的N -糖基化的主要变化可总结如下：1）高甘露糖向复合糖肽的转化大大降低；2）这种转化降低可能是Man9 - 8 - GlcNAc2 - Asn高甘露糖种类积累的结果。本研究通过比较指数生长的HT - 29 Glc -和HT - 29 Glc +细胞中N -聚糖加工的实际状态，研究了这些N -聚糖加工变化是在细胞培养过程中逐渐出现还是在培养开始时就已存在。在这些条件下，HT - 29 Glc -细胞不表现出任何分化特征。无论所研究的生长阶段如何，HT - 29 Glc +细胞中高甘露糖向复合糖蛋白的转化都严重降低。相比之下，HT - 29 Glc -细胞在两个生长阶段均显示出正常的N -聚糖加工模式。因此，我们得出结论，N -聚糖加工可作为HT - 29细胞肠上皮细胞分化过程的早期生化标志物。
