Urban J H, Miller M A, Drake C T, Dorsa D M
Geriatric Research, Education and Clinical Center, VA Medical Center, Seattle, WA 98108.
J Chem Neuroanat. 1990 Jul-Aug;3(4):277-83.
Vasopressin (AVP)-immunoreactive cells have been previously reported in the medial amygdala (AME) and in the locus coeruleus (LC). The present study was designed to verify the presence of AVP-synthesizing neurons in these areas using in situ hybridization histochemistry. A 35S-labelled oligonucleotide probe, complementary to the glycopeptide portion of the vasopressin-encoding mRNA, was used to label cells expressing the AVP gene in brain sections from male Wistar rats. AVP mRNA-positive cells were identified in the AME and were located throughout the anterodorsal and posterodorsal aspect of the nucleus. Cells in the LC, however, did not exhibit labelling for the glycopeptide portion of the AVP gene. The highest density of labelled cells in the medial amygdala occurred 2.30 to 2.80 mm caudal to bregma. The labelling intensity of the cells averaged 53.8 +/- 3.9 grains/cells and was constant throughout the rostro-caudal extent of the AME. These data demonstrate the presence of AVP-synthesizing cells in the AME and provide a method for quantifying their activity. In addition, these data suggest that the cells in the LC may not synthesize vasopressin.
先前已有报道，血管加压素（AVP）免疫反应性细胞存在于内侧杏仁核（AME）和蓝斑（LC）中。本研究旨在通过原位杂交组织化学技术验证这些区域中合成AVP的神经元的存在。使用与血管加压素编码mRNA的糖肽部分互补的35S标记寡核苷酸探针，标记雄性Wistar大鼠脑切片中表达AVP基因的细胞。在内侧杏仁核中鉴定出了AVP mRNA阳性细胞，这些细胞位于整个核的前背侧和后背侧。然而，蓝斑中的细胞未显示出AVP基因糖肽部分的标记。内侧杏仁核中标记细胞的最高密度出现在脑回尾侧2.30至2.80毫米处。细胞的标记强度平均为53.8 +/- 3.9颗粒/细胞，并且在内侧杏仁核的整个前后范围内保持恒定。这些数据证明了内侧杏仁核中存在合成AVP的细胞，并提供了一种量化其活性的方法。此外，这些数据表明蓝斑中的细胞可能不合成血管加压素。
