Stojanović N, Ruvidić R, Jovcić G, Mijović A
Institute for Medical Research, Bulevar JNA 10/11, Belgrade, Yugoslavia.
Biomed Pharmacother. 1990;44(3):181-4. doi: 10.1016/0753-3322(90)90007-v.
Bone marrow CFU-GM and cluster forming cells were studied in ten patients in different stages of drug-induced agranulocytosis using a methylcellulose cell culture technique in vitro. In the aplastic phase of the disease (A), the number of both CFU-GM and cluster forming cells was decreased in comparison to normal values. In the regenerative phase of the disease (R), the number of both granulocytic progenitors increased but did not reach normal values. In patients considered to be recovered from acute agranulocytosis (Rec), a decreased number of progenitors persisted indicating residual damage at this granulocytic cell level. It is suggested that agranulocytosis is due to isolated damage of granulocytic cells and predictable cascade of events within different cell compartments could be used as an in vivo model for investigation of the regulation of granulopoiesis.
采用体外甲基纤维素细胞培养技术，对10例处于药物性粒细胞缺乏症不同阶段的患者的骨髓CFU - GM和集落形成细胞进行了研究。在疾病的再生障碍期（A），与正常值相比，CFU - GM和集落形成细胞的数量均减少。在疾病的再生期（R），粒细胞祖细胞数量均增加，但未达到正常值。在被认为已从急性粒细胞缺乏症恢复的患者（Rec）中，祖细胞数量持续减少，表明在这个粒细胞水平存在残留损伤。提示粒细胞缺乏症是由于粒细胞的孤立性损伤所致，不同细胞区室内可预测的事件级联可作为体内模型用于研究粒细胞生成的调控。
