College of Environmental Science and Engineering, Tianjin Key Laboratory of Urban Ecology Environmental Remediation and Pollution Control, Nankai University, Tianjin 300071, PR China.
Chemosphere. 2013 Nov;93(9):2101-7. doi: 10.1016/j.chemosphere.2013.07.054. Epub 2013 Aug 22.
Chlorinated phenols and perfluoroalkyl acids (PFAAs) are two kinds of pollutants which are widely present in the environment. Considering liver is the primary toxic target organ for these two groups of chemicals, it is interesting to evaluate the possible joint effects of them on liver. In this work, the combined toxicity of pentachlorophenol (PCP) and perfluorooctane sulfonate (PFOS) or perfluorooctanoic acid (PFOA) were investigated using HepG2 cells. The results indicated that PFOS and PFOA could strengthen PCP's hepatotoxicity. Further studies showed that rather than intensify the oxidative stress or promote the biotransformation of PCP, PFOS (or PFOA) might lead to strengthening of the oxidative phosphorylation uncoupling of PCP. By measuring the intracellular PCP concentration and the cell membrane properties, it was suggested that PFOS and PFOA could disrupt the plasma membrane and increase the membrane permeability. Thus, more cellular accessibility of PCP was induced when they were co-exposed to PCP and PFOS (or PFOA), leading to increased cytotoxicity. Further research is warranted to better understand the combined toxicity of PFAAs and other environmental pollutants.
氯代酚和全氟烷基酸（PFAAs）是两种广泛存在于环境中的污染物。鉴于肝脏是这两类化学物质的主要毒性靶器官，评估它们对肝脏的可能联合效应很有趣。在这项工作中，使用 HepG2 细胞研究了五氯苯酚（PCP）与全氟辛烷磺酸（PFOS）或全氟辛酸（PFOA）的联合毒性。结果表明，PFOS 和 PFOA 可以增强 PCP 的肝毒性。进一步的研究表明，PFOS（或 PFOA）并没有加剧氧化应激或促进 PCP 的生物转化，而是可能导致 PCP 的氧化磷酸化解偶联增强。通过测量细胞内 PCP 浓度和细胞膜特性，提示 PFOS 和 PFOA 可以破坏质膜并增加膜通透性。因此，当它们与 PCP 共同暴露时，会诱导更多的 PCP 进入细胞，导致细胞毒性增加。需要进一步的研究来更好地了解 PFAAs 与其他环境污染物的联合毒性。
