State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100083, China.
J Chromatogr A. 2013 Sep 27;1309:90-5. doi: 10.1016/j.chroma.2013.08.026. Epub 2013 Aug 14.
Thirty-eight short peptaibols in a microheterogeneous mixture derived from the fermentation broth of a marine-derived fungus Trichoderma asperellum were determined using ultrahigh pressure liquid chromatography in combination with electrospray-ionization tandem mass spectrometry (UHPLC-ESIMS/MS) techniques, including thirty-two new peptaibols namely asperelines G-Z13. The C-terminus bonded to proline (aspereline Z9) or hydroxyprolinol (aspereline Z12) is rarely found in nature. So far, it is the largest number of peptaibols to be detected at once on the basis of the selected ion monitoring (SIM) mode coupled to precursor scan techniques.
采用超高压液相色谱-电喷雾串联质谱联用技术（UHPLC-ESI-MS/MS），从海洋来源真菌 Aspergillus terreus 的发酵液中分离得到了 38 种短肽类化合物组成的微不均一混合物，其中包括 32 种新的短肽类化合物，命名为 asperelines G-Z13。C 端与脯氨酸（aspereline Z9）或羟脯氨酸（aspereline Z12）键合的结构在自然界中很少见。到目前为止，这是基于与前体扫描技术相结合的选择离子监测（SIM）模式下一次检测到的最大数量的肽类化合物。
