School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea.
Colloids Surf B Biointerfaces. 2013 Dec 1;112:134-8. doi: 10.1016/j.colsurfb.2013.07.021. Epub 2013 Jul 18.
Protein micropatterned surfaces integrated with microfluidics are useful in numerous bioanalytical and biological applications. In this study, we demonstrated the fabrication of complex protein micropatterned surfaces within poly(dimethylsiloxane) (PDMS) microfluidic channels by attaching the PDMS channels to bio-friendly photoresist films and subsequently performing microscope projection photolithography (MPP). A muscle-inspired poly(dopamine) (PDA) coating was employed to mediate the bonding between the PDMS and the bio-friendly photoresist poly(2,2-dimethoxy nitrobenzyl methacrylate-r-methyl methacrylate-r-poly(ethylene glycol) methacrylate) (PDMP). By adjusting the dip-coating time for the PDA coating, we could successfully introduce sufficient amounts of functional groups on the PDMP surfaces to mediate strong bonding between the PDMS channels and the PDA-coated PDMP thin films with minimal alteration of the surface properties of the PDMP thin films that are critical for protein micropatterning. Using this novel bonding strategy, we successfully fabricated multiple protein micropatterns and gradient micropatterns of proteins within microfluidic channels. The technique developed in this study will be useful for the fabrication of complex biochips for multiplex bioassays and fundamental cell biological studies.
蛋白质微图案表面与微流控相结合在许多生物分析和生物学应用中非常有用。在这项研究中，我们通过将 PDMS 通道附着到生物友好的光致抗蚀剂薄膜上，并随后进行显微镜投影光刻（MPP），展示了在 PDMS 微流道内制造复杂蛋白质微图案表面的方法。受肌肉启发的聚多巴胺（PDA）涂层被用来介导 PDMS 和生物友好的光致抗蚀剂聚（2,2-二甲氧基硝基苯甲基甲基丙烯酸酯-r-甲基丙烯酸甲酯-r-聚（乙二醇）甲基丙烯酸酯）（PDMP）之间的键合。通过调整 PDA 涂层的浸渍时间，我们可以成功地在 PDMP 表面引入足够数量的官能团，以在 PDMS 通道和 PDA 涂覆的 PDMP 薄膜之间形成强键合，同时最小化 PDMP 薄膜表面性能的变化，这对于蛋白质微图案化至关重要。使用这种新的键合策略，我们成功地在微流道内制造了多种蛋白质微图案和蛋白质的梯度微图案。本研究中开发的技术将有助于制造用于多重生物分析和基础细胞生物学研究的复杂生物芯片。
