Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou 510642, China; Zhongkai University of Agriculture and Engineering, College of Life Sciences, China.
Microb Pathog. 2013 Nov;64:39-42. doi: 10.1016/j.micpath.2013.08.001. Epub 2013 Aug 22.
Pigs are susceptible to both human and avian influenza viruses (AIV). Moreover, they are suspected of being the intermediate hosts or mixing vessels of pandemic influenza viruses. Researchers suspect that the influenza viruses are able to undergo reassortment or to adapt to various mammalian hosts while they incubate in pigs. For the present report, we conducted a serological surveillance of pigs in southern China from 2008 to 2012 to establish the prevalence of antibodies against H5N1 and H9N2 AIV. A total of one hundred pig farms from the Guangdong, Zhejiang, Fujian, and Yunnan Provinces were sampled, yielding a total of 3960 serum specimens. The haemagglutination inhibition (HI) tests revealed no evidence of H5 infection when the Clade 2.3.2 virus was used as the antigen, but a 4.6% positive rate of H9 infection was observed when using the Beijing/1/94-like virus as the antigen. The positive sera for H9 infection were further verified with neutralization tests, which confirmed a 3.7% rate of positive sera of H9 infection. In summary, the results imply that the swine populations in southern China had not been affected greatly by the H5N1 avian influenza virus. Nevertheless, these swine H9N2 influenza viruses might pose a threat to human health, and so researchers should continue to carry out swine influenza virus surveillance in China.
猪既容易感染人流感病毒，也容易感染禽流感病毒（AIV）。此外，它们被怀疑是大流行性流感病毒的中间宿主或混合宿主。研究人员怀疑流感病毒在猪体内孵育时能够发生重配或适应各种哺乳动物宿主。在本报告中，我们对 2008 年至 2012 年中国南方的猪群进行了血清学监测，以确定对 H5N1 和 H9N2 AIV 的抗体流行率。从广东、浙江、福建和云南省共采集了 100 个猪场的样本，共获得 3960 份血清样本。当使用 Clade 2.3.2 病毒作为抗原时，血凝抑制（HI）试验未发现 H5 感染的证据，但当使用北京/1/94 样病毒作为抗原时，H9 感染的阳性率为 4.6%。对 H9 感染的阳性血清进一步进行中和试验验证，证实 H9 感染的阳性血清率为 3.7%。总之，结果表明中国南方的猪群没有受到 H5N1 禽流感病毒的严重影响。然而，这些猪 H9N2 流感病毒可能对人类健康构成威胁，因此研究人员应继续在中国开展猪流感病毒监测。
