Chen Jidang, Ma Jun, White Sarah K, Cao Zhenpeng, Zhen Yun, He Shuyi, Zhu Wanjun, Ke Changwen, Zhang Yongbiao, Su Shuo, Zhang Guihong
College of Veterinary and National Engineering Research Center for Breeing Swine Industry/South China Agricultural University, Guangzhou, Guangdong Province, PR China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, Guangdong Province, PR China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, Guangdong Province, PR China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangdong Center for Disease Control and Prevention, Guangzhou, Guangdong Province, PR China.
Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, USA.
Vet Microbiol. 2015 Dec 31;181(3-4):230-5. doi: 10.1016/j.vetmic.2015.09.016. Epub 2015 Sep 25.
Guangdong Province is recognized for dense populations of humans, pigs, poultry and pets. In order to evaluate the threat of viral infection faced by those working with animals, a cross-sectional, sero-epidemiological study was conducted in Guangdong between December 2013 and January 2014. Individuals working with swine, at poultry farms, or live poultry markets (LPM), and veterinarians, and controls not exposed to animals were enrolled in this study and 11 (4 human, 3 swine, 3 avian, and 1 canine) influenza A viruses were used in hemagglutination inhibition (HI) assays (7 strains) and the cross-reactivity test (9 strains) in which 5 strains were used in both tests. Univariate analysis was performed to identify which variables were significantly associated with seropositivity. Odds ratios (OR) revealed that swine workers had a significantly higher risk of elevated antibodies against A/swine/Guangdong/L6/2009(H1N1), a classical swine virus, and A/swine/Guangdong/SS1/2012(H1N1), a Eurasian avian-like swine virus than non-exposed controls. Poultry farm workers were at a higher risk of infection with avian influenza H7N9 and H9N2. LPM workers were at a higher risk of infection with 3 subtypes of avian influenza, H5N1, H7N9, and H9N2. Interestingly, the OR also indicated that LPM workers were at risk of H1N1 swine influenza virus infection, perhaps due to the presence of pigs in the LPM. While partial confounding by cross-reactive antibodies against human viruses or vaccines cannot be ruled out, our data suggests that animal exposed people as are more likely to have antibodies against animal influenza viruses.
广东省以人口、猪、家禽和宠物密集而闻名。为了评估与动物接触的人员所面临的病毒感染威胁，于2013年12月至2014年1月在广东省开展了一项横断面血清流行病学研究。参与本研究的人员包括在养猪场、家禽养殖场或活禽市场工作的人员、兽医，以及未接触动物的对照人员，并使用了11株（4株人源、3株猪源、3株禽源和1株犬源）甲型流感病毒进行血凝抑制（HI）试验（7株病毒）和交叉反应试验（9株病毒），其中5株病毒用于两项试验。进行单因素分析以确定哪些变量与血清阳性显著相关。比值比（OR）显示，养猪场工人针对经典猪病毒A/猪/广东/L6/2009（H1N1）和欧亚禽源类猪病毒A/猪/广东/SS1/2012（H1N1）抗体升高的风险显著高于未接触动物的对照人员。家禽养殖场工人感染甲型H7N9和H9N2流感的风险较高。活禽市场工作人员感染甲型H5N1、H7N9和H9N2三种亚型禽流感的风险较高。有趣的是，OR值还表明，活禽市场工作人员有感染甲型H1N1猪流感病毒的风险，这可能是由于活禽市场中有猪存在。虽然不能排除针对人类病毒或疫苗的交叉反应抗体造成的部分混杂因素，但我们的数据表明，接触动物的人群更有可能拥有针对动物流感病毒的抗体。
