Department of Microbiology and Biotechnology, Federal Research Institute of Nutrition and Food, Max Rubner-Institute, Hermann-Weigmannstraße 1, 24103, Kiel, Germany,
Antonie Van Leeuwenhoek. 2013 Dec;104(6):925-31. doi: 10.1007/s10482-013-0011-5. Epub 2013 Aug 23.
Five novel ascosporogenous yeast strains (H382, H396, H409, H433(T) and H441) were found through a survey of vacuum-packed beef microbiota. Sequence analysis of ITS domain and LSU rRNA genes showed that the new strains represent a distinct lineage within the genus Kazachstania, closely related to Kazachstania lodderae (97.0 % identity) and Kazachstania ichnusensis (96.1 % identity). The main difference of strains H382, H396, H409, H433(T) and H441 to strains of known Kazachstania species is the maximum growth temperature, which is below 20 °C for the new strains, whereas related species grow at 25 °C. Furthermore, the strains differed from known Kazachstania species in assimilation and fermentation patterns of carbon sources. Based on these characteristics, the five strains are considered to represent a novel species of the genus Kazachstania for which the name Kazachstania psychrophila sp. nov. is proposed. The type strain is H433(T) (DSM 26230(T)=CBS 12689(T)). The Mycobank number of the type strain is MB 803980.
通过对真空包装牛肉微生物群的调查，发现了五株新型的子囊孢子形成酵母菌株（H382、H396、H409、H433(T) 和 H441）。ITS 区和 LSU rRNA 基因序列分析表明，这些新菌株代表了 Kazachstania 属中的一个独特谱系，与 Kazachstania lodderae（97.0%同源性）和 Kazachstania ichnusensis（96.1%同源性）密切相关。与已知的 Kazachstania 种相比，菌株 H382、H396、H409、H433(T) 和 H441 的主要区别在于最大生长温度，新菌株的最大生长温度低于 20°C，而相关物种在 25°C 下生长。此外，这些菌株在碳源同化和发酵模式上与已知的 Kazachstania 物种不同。基于这些特征，这五株菌株被认为代表了 Kazachstania 属的一个新种，命名为 Kazachstania psychrophila sp. nov.。模式菌株为 H433(T)（DSM 26230(T)=CBS 12689(T)）。模式菌株的 MycoBank 编号为 MB 803980。
