Di Mauro C, Cavalli G, Amprimo M C, Paradisi L, Scano G, Curzio M, Dianzani M U
Dipartimento di Medicina ed Oncologia Sperimentale, Sezione di Patologia Generale, Torino, Italy.
Cell Biochem Funct. 1990 Jul;8(3):147-55. doi: 10.1002/cbf.290080304.
The lipid peroxidation product 4-hydroxy-2, 3-trans-nonenal (HNE) has a spectrum of biological effects on different cell types depending on the concentrations tested. In particular micromolar HNE concentrations stimulate neutrophil migration and polarization whereas higher doses inhibit. In our experimental conditions, fMet-Leu-Phe (fMLP) increased CL production of both unstimulated and zymosan-stimulated neutrophils, whereas cell stimulation with low HNE concentrations as well as zymosan addition to HNE incubated cells did not enhance light emission. In contrast 10(-4) M HNE reduced CL emission by unstimulated cells nearly to background values, completely depressed CL production by zymosan-stimulated cells and reduced phagocytosis. Cysteine was found to be able to counteract the HNE effect by about 70 per cent. The possibility that this aldehyde could exert its inhibitory effect through the alkylation of NADPH-oxidase SH-groups is postulated. Moreover, our present data on differences observed between fMLP and HNE indicate a different chemotactic mechanism induced by these two classes of compounds and lead to the conclusion that the local functional features of the attracted cells may be different.
脂质过氧化产物4-羟基-2,3-反式壬烯醛（HNE）根据所测试的浓度对不同细胞类型具有一系列生物学效应。特别是微摩尔浓度的HNE刺激中性粒细胞迁移和极化，而更高剂量则起抑制作用。在我们的实验条件下，甲酰甲硫氨酸-亮氨酸-苯丙氨酸（fMLP）增加了未刺激和经酵母聚糖刺激的中性粒细胞的化学发光（CL）产生，而低浓度HNE刺激细胞以及向经HNE孵育的细胞中添加酵母聚糖均未增强发光。相反，10^(-4) M HNE将未刺激细胞的CL发射降低至几乎背景值，完全抑制了酵母聚糖刺激细胞的CL产生并降低了吞噬作用。发现半胱氨酸能够抵消约70%的HNE效应。推测这种醛可能通过对NADPH氧化酶的巯基进行烷基化来发挥其抑制作用。此外，我们目前关于fMLP和HNE之间差异的数据表明这两类化合物诱导了不同的趋化机制，并得出结论，被吸引细胞的局部功能特征可能不同。
