An Chung-Ii, Hagiwara Nobuko
Division of Cardiovascular Medicine, Department of Internal Medicine, University of California, Davis, Davis, CA, USA.
Methods Mol Biol. 2013;1067:51-64. doi: 10.1007/978-1-62703-607-8_4.
Transcriptional regulation of gene expression constitutes a fundamental mechanism of many developmental processes. Therefore, identification and characterization of binding sites of transcription factors are important for uncovering the mechanisms of a particular developmental process. Here, we describe detailed procedures for genome-wide analysis of binding sites of a transcription factor involved in the fiber-type differentiation of skeletal muscle. By conducting ChIP-seq followed by a series of downstream analyses, in-depth information on binding sites of transcription factors can be obtained in a genome-wide manner.
基因表达的转录调控是许多发育过程的基本机制。因此，鉴定和表征转录因子的结合位点对于揭示特定发育过程的机制很重要。在这里，我们描述了用于全基因组分析参与骨骼肌纤维类型分化的转录因子结合位点的详细程序。通过进行ChIP-seq并随后进行一系列下游分析，可以以全基因组方式获得关于转录因子结合位点的深入信息。
