Detection of apolipoprotein E polymorphisms using PCR/ASO probes and Southern transfer: application for routine use. - Suppr | 超能文献

Detection of apolipoprotein E polymorphisms using PCR/ASO probes and Southern transfer: application for routine use.

作者信息

Houlston R S, Wenham P R, Humphries S E

机构信息

Arterial Diseases Research Group, Charing Cross Sunley Research Centre, London, UK.

出版信息

Clin Chim Acta. 1990 Aug 15;189(2):153-7. doi: 10.1016/0009-8981(90)90086-8.

DOI:10.1016/0009-8981(90)90086-8

Abstract

The detection of apolipoprotein E genotypes is of importance both for diagnostic and research purposes. We have previously used the polymerase chain reaction to amplify a specific region of the apolipoprotein E gene which, when used in conjunction with allele specific oligonucleotide probes, permits the detection of the six common apolipoprotein genotypes. In our present report we have modified the above procedure by immobilising the amplified DNA using Southern blotting. This enables the technique to be used in routine laboratories with minimal expenditure and little risk of cross-contamination between samples. Furthermore, it is inherently robust and may be rapidly performed.

摘要

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