灵长类动物（埃及狒狒）精子发生的脱氧核糖核酸流式细胞术分析的最佳睾丸抽吸技术。

Optimum testicular aspiration technique for deoxyribonucleic acid flow cytometric analysis of spermatogenesis in primates (Papio anubis).

作者信息

Hellstrom W J, Kaack B

机构信息

Department of Urology, Delta Regional Primate Research Center, Tulane University School of Medicine, New Orleans, Louisiana 70112.

出版信息

Fertil Steril. 1990 Sep;54(3):517-21.

PMID:2397795

Abstract

Deoxyribonucleic acid flow cytometry on testicular tissue is an established method to evaluate spermatogenesis. Needle aspiration is less invasive and permits sequential sampling as compared with open testis biopsy. Using seven primates, we examined the needle aspiration technique. There was no significant difference among locations aspirated; the Westcott needle (Bard Urological, Covington, GA) provided the most rapid analysis, and both the Biopty gun (Bard Urological) and Westcott needle were easy to use. Baseline means +/- SD for ploidy distributions were 1N: 65.73% +/- 7.1; 2N: 18.95% +/- 5.5; 4N: 11.4% +/- 2, Experimental data generated from primate models using testis aspiration and flow cytometry may elucidate human infertile conditions.

摘要

对睾丸组织进行脱氧核糖核酸流式细胞术是评估精子发生的一种既定方法。与开放性睾丸活检相比，针吸活检侵入性较小，且允许进行连续采样。我们使用七只灵长类动物对针吸技术进行了研究。所抽吸的不同部位之间没有显著差异；韦斯科特针（巴德泌尿外科公司，佐治亚州科文顿）提供了最快的分析速度，并且活检枪（巴德泌尿外科公司）和韦斯科特针都易于使用。倍性分布的基线均值±标准差为1N：65.73%±7.1；2N：18.95%±5.5；4N：11.4%±2。使用睾丸针吸活检和流式细胞术从灵长类动物模型生成的实验数据可能有助于阐明人类的不育状况。