Clinical Protein Science & Imaging, Biomedical Center, Department of Measurement Technology and Industrial Electrical Engineering, Lund University , BMC C13, 221 84 Lund, Sweden.
J Proteome Res. 2013 Oct 4;12(10):4612-6. doi: 10.1021/pr400423k. Epub 2013 Sep 9.
Recently, the number of mass spectrometry-based quantification assays has been increased, partially due to the global efforts of chromosome-centric human proteome project (C-HPP). Our goal at the Chromosome 19 Consortium is to provide novel selected reaction monitoring (SRM) assays of proteins coded on chromosome 19. We have selected the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR) of human kallikrein-2 (hK2 - NX_P20151) and developed an SRM assay. Details of the analytical parameters, including multiple transitions by peptides, are presented. The endogenous levels of hK2 were determined in clinical samples (n = 35). The limit of quantification was also estimated by spiking heavy isotope-labeled peptides into seminal plasma samples at various concentrations (LOQ ≈ 29 ng/mL).
最近，基于质谱的定量分析检测方法的数量有所增加，这部分归因于全人类染色体蛋白质组计划（C-HPP）的全球努力。我们 19 号染色体联盟的目标是提供 19 号染色体编码的蛋白质的新型选择反应监测（SRM）检测方法。我们选择了人类激肽释放酶-2（hK2-NX_P20151）的两个最有用的特征肽（NSQVWLGR 和 HNLFEPEDTGQR），并开发了一种 SRM 检测方法。呈现了分析参数的详细信息，包括肽的多个转换。在临床样本（n = 35）中测定了 hK2 的内源性水平。通过在不同浓度下将重同位素标记的肽掺入精液样本中来估计定量下限（LOQ≈29ng/mL）。
