嗜酸热原体 Thermoplasma acidophilum 的首次转化方法。
The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum.
机构信息
Department of Environmental Protection & Environmental Safety, Szent István University, Gödöllő 2100, Hungary; Department of Microbiology, Central Environmental and Food Science Research Institute, Budapest 1022, Hungary.
出版信息
J Microbiol Methods. 2013 Nov;95(2):145-8. doi: 10.1016/j.mimet.2013.08.006. Epub 2013 Aug 23.
A transformation method yielding up to 10(4) transformants per μg circular DNA was developed for Thermoplasma acidophilum. The method is based on a natural DNA uptake process in which T. acidophilum cells keep their integrity and turn competent at pH 3.5 and 58°C. Shuttle vector maintenance could not be detected, since the used Nov(R) gyraseB gene integrated into its chromosomal counterpart by homologous recombination.
一种可使嗜酸性热原体每微克环状 DNA 产生多达 10(4)个转化体的转化方法被开发出来。该方法基于一种天然的 DNA 摄取过程，在此过程中，嗜酸性热原体细胞保持其完整性，并在 pH 3.5 和 58°C 时变得具有感受态。由于所用的 Nov(R)gyraseB 基因通过同源重组整合到其染色体对应物中，因此无法检测到穿梭载体的维持。
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