小鼠原代胰腺腺泡细胞的分离与培养。
Isolation and culture of mouse primary pancreatic acinar cells.
作者信息
Gout Johann, Pommier Roxane M, Vincent David F, Kaniewski Bastien, Martel Sylvie, Valcourt Ulrich, Bartholin Laurent
机构信息
INSERM U1052, Centre de Recherche en Cancérologie de Lyon.
出版信息
J Vis Exp. 2013 Aug 13(78):50514. doi: 10.3791/50514.
Abstract
This protocol permits rapid isolation (in less than 1 hr) of murine pancreatic acini, making it possible to maintain them in culture for more than one week. More than 20 x 10(6) acinar cells can be obtained from a single murine pancreas. This protocol offers the possibility to independently process as many as 10 pancreases in parallel. Because it preserves acinar architecture, this model is well suited for studying the physiology of the exocrine pancreas in vitro in contrast to cell lines established from pancreatic tumors, which display many genetic alterations resulting in partial or total loss of their acinar differentiation.
摘要
该方案可快速分离（不到1小时）小鼠胰腺腺泡，使其能够在培养中维持一周以上。从单个小鼠胰腺可获得超过20×10⁶个腺泡细胞。该方案提供了同时独立处理多达10个胰腺的可能性。由于它保留了腺泡结构，与从胰腺肿瘤建立的细胞系相比，该模型非常适合在体外研究外分泌胰腺的生理学，胰腺肿瘤细胞系显示出许多基因改变，导致其腺泡分化部分或完全丧失。
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