Pró-Reitoria de Pós-Graduação, Pesquisa e Extensão, Universidade CEUMA (UNICEUMA), Rua Josué Montello, 1, São Luís, Maranhão, 65075-120, Brazil.
Inflamm Res. 2013 Nov;62(11):971-80. doi: 10.1007/s00011-013-0659-3. Epub 2013 Aug 25.
Among the options for treatment of diseases affecting the respiratory system, especially asthma, drug delivering systems for intranasal application represent an important therapeutic approach at the site of inflammation. The present study aimed to evaluate the therapeutic effect of biodegradable microparticles formed by poly lactic-co-glycolic acid (PLGA) containing encapsulated pomegranate extract on a murine model of asthma.
The extract was acquired from the leaves of P. granatum and characterized qualitatively by HPLC. A w/o/w emulsion solvent extraction-evaporation method was chosen to prepare the microparticles containing pomegranate encapsulated extract (MP).
OVA-sensitized BALB/c mice were used as asthma model and treated with dexamethasone and P. granatum extract in solution form or encapsulated into microparticles.
MP were able to inhibit leukocytes' recruitment to bronchoalveolar fluid, especially, eosinophils, decreasing cytokines (IL-1β and IL-5) and protein levels in the lungs.
This approach can be used as an alternative/supplementary therapy based on the biological effects of P. granatum for managing inflammatory processes, especially those with pulmonary complications.
在治疗呼吸系统疾病（尤其是哮喘）的各种方法中，鼻腔内给药的药物输送系统是一种在炎症部位发挥重要治疗作用的方法。本研究旨在评估载有石榴提取物的聚乳酸-共-羟基乙酸（PLGA）可生物降解微球对哮喘小鼠模型的治疗效果。
从石榴叶中提取的提取物，并用 HPLC 进行定性分析。采用 w/o/w 乳液溶剂萃取-蒸发法制备载有石榴提取物的微球（MP）。
卵清蛋白（OVA）致敏的 BALB/c 小鼠被用作哮喘模型，并用地塞米松和以溶液形式或封装成微球形式的石榴提取物进行治疗。
MP 能够抑制白细胞向支气管肺泡液中的募集，特别是抑制嗜酸性粒细胞，并降低肺部细胞因子（IL-1β 和 IL-5）和蛋白质的水平。
这种方法可作为基于石榴的生物学效应的替代/补充疗法，用于治疗炎症过程，特别是那些伴有肺部并发症的炎症过程。
