Zhang J, Sun X L, Zhang L G, Hui M X, Zhang M K
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
Genet Mol Res. 2013 Jul 24;12(3):2507-16. doi: 10.4238/2013.July.24.5.
Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA occurs frequently, its mechanism remains unknown. To elucidate the molecular mechanism underlying FBA, we detected gene expression differences between aborted and normal buds of radish using cDNA-amplified fragment length polymorphism (AFLP) and real-time polymerase chain reaction (real-time PCR). A total of 221 differentially expressed transcript-derived fragments (TDFs) were detected by 256 cDNA-AFLP primer combinations, of which 114 were upregulated and 107 were downregulated in the aborted buds. A total of 54 TDFs were cloned and sequenced. A BLAST search revealed that all TDFs have homologous sequences and 29 of these corresponded to known genes, whose functions were mainly related to metabolism, stimulus response, transcriptional regulation, and transportation. Expressions of 6 TDFs with different functions were further analyzed by real-time PCR yielding expression profiling results consistent with the cDNA-AFLP analysis. Our results indicated that radish FBA is related to abnormalities in various physiological and biochemical plant processes.
萝卜花芽败育（FBA）是一种在生殖过程中出现的不良生物学现象。尽管FBA频繁发生，但其机制仍不清楚。为了阐明FBA潜在的分子机制，我们利用cDNA扩增片段长度多态性（AFLP）和实时聚合酶链反应（实时PCR）检测了萝卜败育花芽与正常花芽之间的基因表达差异。通过256对cDNA-AFLP引物组合共检测到221个差异表达的转录衍生片段（TDF），其中114个在败育花芽中上调，107个下调。共克隆并测序了54个TDF。BLAST搜索显示所有TDF均有同源序列，其中29个与已知基因对应，其功能主要与代谢、刺激响应、转录调控和运输有关。通过实时PCR进一步分析了6个具有不同功能的TDF的表达情况，得到的表达谱结果与cDNA-AFLP分析一致。我们的结果表明，萝卜FBA与植物各种生理生化过程的异常有关。
