[丝裂原活化蛋白激酶信号通路在复方丹参注射液诱导人羊膜上皮细胞水通道蛋白3表达调控中的作用]

[The role of MAPK signal pathway in the regulation of AQP3 expression induced by compound danshen injection in human amniotic epithelial cells].

作者信息

Ma Xiao-Yan, Shen Qi, Hua Ying, Xie Ai-Lan, Zhu Xue-Qiong

机构信息

Department of Obstetrics and Gynecology, Second Affiliated Hospital of Wenzhou Medical College, Zhejiang 325027, China.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 Jun;33(6):778-82.

PMID:23980358

Abstract

OBJECTIVE

To investigate the role of mitogen-activated protein kinases (MAPKs)-extracellular signal regulated kinase1/2 (ERK1/2) signal pathway in the regulation of Compound Danshen Injection (CDI) induced AQP3 expression in the human amniotic epithelial cells (hAECs).

METHODS

hAECs of term pregnancy with normal amniotic fluid volume (AFV) or isolated oligohydramnios were primarily cultured. And the cells were equally divided into four groups, i.e., the vehicle control group, the U0126 group, the CDI group, the CDI + U0126 group. The expressions of phosphorylated ERK1/2 (p-ERK1/2) and AQP3 in hAECs were detected using Western blot analysis.

RESULTS

(1) When compared with the control group, the expression level of p-ERK1/2 in hAECs in those with normal AFV and oligohydramnios obviously decreased in the U0126 group (P < 0.05). The expression level of p-ERK1/2 could be elevated in the CDI group (P < 0.05). The expression level of p-ERK1/2 in hAECs was higher in the CDI +U0126 group than in the U0126 group, but lower in the CDI + U0126 group than in the CDI group (P < 0.05). (2) There was no obvious change in AQP3 expression in hAECs with normal AFV between the U0126 group and the vehicle control group (P > 0.05). There was no statistical difference in the expression level of AQP3 between the CDI group and the U0126 +CDI group (P > 0.05), but they were higher than those in the vehicle control group (P < 0.05). (3) Compared with the vehicle control group, the expression level of AQP3 in hAECs with oligohydramnios significantly decreased in the U0126 group and increased in the CDI group (P < 0.05). The expression level of AQP3 was lower in the U0126 + CDI group than in the CDI group, but higher in the U0126 +CDI group than in the U0126 group (P < 0.05).

CONCLUSION

CDI could regulate AQP3 expression level in hAECs with oligohydramnios via activating the MAPK-ERK1/2 signal transduction pathway.

摘要

目的

探讨丝裂原活化蛋白激酶（MAPKs）-细胞外信号调节激酶1/2（ERK1/2）信号通路在复方丹参注射液（CDI）诱导人羊膜上皮细胞（hAECs）水通道蛋白3（AQP3）表达调控中的作用。

方法

对羊水正常或孤立性羊水过少的足月妊娠hAECs进行原代培养。将细胞平均分为四组，即溶剂对照组、U0126组、CDI组、CDI + U0126组。采用蛋白质免疫印迹法检测hAECs中磷酸化ERK1/2（p-ERK1/2）和AQP3的表达。

结果

（1）与对照组相比，U0126组羊水正常和羊水过少的hAECs中p-ERK1/2表达水平明显降低（P < 0.05）。CDI组p-ERK1/2表达水平升高（P < 0.05）。CDI + U0126组hAECs中p-ERK1/2表达水平高于U0126组，但低于CDI组（P < 0.05）。（2）U0126组与溶剂对照组羊水正常的hAECs中AQP3表达无明显变化（P > 0.05）。CDI组与U0126 + CDI组AQP3表达水平无统计学差异（P > 0.05），但均高于溶剂对照组（P < 0.05）。（3）与溶剂对照组相比，U0126组羊水过少的hAECs中AQP3表达明显降低而CDI组升高（P < 0.05）。U0126 + CDI组AQP3表达水平低于CDI组，但高于U0126组（P < 0.05）。