Shen Qi-Ming, Ma Li-Hong, Wang Shao-Xia, Li Yang, Zhang Rui-Hua
Department of Traditional Chinese Medicine, Fuwai Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100037, China.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 Jun;33(6):783-8.
To investigate the effects of Xinfuli Granule (XG) on cardiomyocyte apoptosis in rats with adriamycin-induced dilated cardiomyopathy (DCM).
Seventy-two male SD rats were randomly divided into 6 groups, i.e., the normal control group, the model group, the irbesartan group, the low dose XG group, the medium dose XG group, and the high dose XG group. The DCM heart failure rat model was established using peritoneal injection of ADR. Equal volume of normal saline was injected to those in the normal control group, once per week for 6 consecutive weeks. The medication was started from the 5th week by gastrogavage. XG was dispensed into 0.5 g/mL suspension with distilled water. The XG was administered at the daily dose of 0.675 g/kg, 1.350 g/kg, and 2.700 g/kg to those in the low dose XG group, the medium dose XG group, and the high dose XG group, respectively. Irbesartan was administered to rats in the irbesartan group at the daily dose of 50 mg/kg. Equal volume of normal saline was administered to those in the normal control group and the model group by gastrogavage, once in the morning for 4 consecutive weeks. Myocardial apoptosis was measured by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and the expressions of the Bcl-2 and Bax protein of cardiomyocytes were measured by immunohistochemical assay.
Compared with the normal control group, the cardiomyocyte apoptosis rate and Bax expression level obviously increased, but the expression of Bcl-2 and the Bcl-2/Bax ratio decreased significantly in the model group (P < 0.05). Compared with the model group, the expression of Bax and the Bcl-2/Bax ratio increased significantly in the high dose XG group and the irbesartan group (P < 0.01). The Bax expression level obviously decreased in all groups except the normal control group (P < 0.01).
XG could obviously attenuate cardiomyocyte apoptosis in the adriamycin-induced DCM rats, and reverse the occurrence and development of heart reconstruction. The underlying mechanism might be related to regulating and controlling the expressions of Bax and Bcl-2.
探讨心复力颗粒（XG）对阿霉素诱导的扩张型心肌病（DCM）大鼠心肌细胞凋亡的影响。
将72只雄性SD大鼠随机分为6组，即正常对照组、模型组、厄贝沙坦组、XG低剂量组、XG中剂量组和XG高剂量组。采用腹腔注射阿霉素建立DCM心力衰竭大鼠模型。正常对照组大鼠腹腔注射等体积生理盐水，每周1次，连续6周。从第5周开始灌胃给药。XG用蒸馏水配制成0.5 g/mL的混悬液。XG低剂量组、XG中剂量组和XG高剂量组大鼠分别按每日0.675 g/kg、1.350 g/kg和2.700 g/kg的剂量灌胃给药。厄贝沙坦组大鼠按每日50 mg/kg的剂量灌胃给药。正常对照组和模型组大鼠灌胃等体积生理盐水，每天上午1次，连续4周。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）检测心肌细胞凋亡情况，免疫组化法检测心肌细胞Bcl-2和Bax蛋白的表达。
与正常对照组比较，模型组心肌细胞凋亡率及Bax表达水平明显升高，Bcl-2表达及Bcl-2/Bax比值明显降低（P < 0.05）。与模型组比较，XG高剂量组和厄贝沙坦组Bax表达及Bcl-2/Bax比值明显升高（P < 0.01）。除正常对照组外，其余各组Bax表达水平均明显降低（P < 0.01）。
XG可明显减轻阿霉素诱导的DCM大鼠心肌细胞凋亡，逆转心脏重构的发生发展。其潜在机制可能与调控Bax和Bcl-2的表达有关。
