Faculty of Science, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 6500, Thailand.
J Biomed Nanotechnol. 2013 Sep;9(9):1509-20. doi: 10.1166/jbn.2013.1645.
We herein report the surface modification of magnetite nanoparticle (MNP) with the (co)polymer of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and/or diethylamino ethyl methacrylate (DEAEMA) via atom transfer radical polymerization (ATRP) for use as anion exchanger solid support for detection of DNA sequence using peptide nucleic acid (PNA) probe. Molar ratio of the PEGMA:DEAEMA (co)polymer was systematically varied to tune the positive charges on the particle surface. Kinetic studies of the (co)polymerizations were investigated via 1HNMR to disclose the relative reactivity of the (co)polymers in the reaction. Zeta potential of the (co)polymer-grafted MNP was analyzed by photo correlation spectroscopy (PCS). Transmission electron microscopy (TEM) and PCS indicated an improvement in the particle dispersibility in water upon quaternization of the DEAEMA entities grafted on the particle surface. From the preliminary results, these (co)polymer-grafted MNPs can be used as a nanosolid support to differentiate between full match and single-base mismatch DNA sequences using an acpcPNA probe. These novel cationic MNPs might be efficiently applicable for use as a magnetically guidable tool for detection of DNA sequences.
我们在此报告通过原子转移自由基聚合（ATRP）将聚乙二醇甲基醚甲基丙烯酸酯（PEGMA）和/或二乙氨基乙基甲基丙烯酸酯（DEAEMA）的共聚物修饰到磁铁矿纳米颗粒（MNP）上，用作用于检测使用肽核酸（PNA）探针的 DNA 序列的阴离子交换固相支持。通过 1HNMR 研究聚合动力学，以揭示聚合反应中（共）聚合物的相对反应性，从而系统地改变 PEGMA：DEAEMA（共）聚合物的摩尔比，以调节颗粒表面的正电荷。通过光相关光谱（PCS）分析接枝共聚物的 MNP 的 ζ 电位。透射电子显微镜（TEM）和 PCS 表明，在颗粒表面接枝的 DEAEMA 官能团季铵化后，颗粒在水中的分散性得到改善。从初步结果来看，这些接枝共聚物的 MNPs 可用作纳米固相支持物，使用 acpcPNA 探针区分全匹配和单碱基错配的 DNA 序列。这些新型阳离子 MNPs 可能非常适用于作为用于检测 DNA 序列的磁引导工具。
