Moorman S J, Hume R I
Department of Biology, University of Michigan, Ann Arbor 48109.
J Neurosci. 1990 Sep;10(9):3158-63. doi: 10.1523/JNEUROSCI.10-09-03158.1990.
The ability of the growth cones of sympathetic preganglionic neurons to recognize the neurons they encounter during their outgrowth and to react to them in a cell-type-specific manner may play a role in guiding them to appropriate targets during development in vivo. In this study, we examined the in vitro growth of sympathetic preganglionic neurons as they interacted with motor neurons, dorsal root ganglion neurons, and sympathetic ganglion neurons. All of these cell types might potentially be encountered by a growing preganglionic axon. The interaction of sympathetic preganglionic growth cones with each cell type was distinct. Sympathetic preganglionic growth cones fasciculated on motor-neuron neurites, collapsed after contact with the cell bodies and neurites of dorsal root ganglion neurons, and grew across the cell bodies and neurites of sympathetic ganglion neurons. These cell-type-specific responses stand in contrast to the collapse and retraction reported to be the most common growth-cone behaviors that result from contact between central and peripheral neurons in vitro and suggest that contact-mediated recognition might be sufficient for growth to and interaction with appropriate targets.
交感神经节前神经元的生长锥在生长过程中识别所遇到的神经元并以细胞类型特异性方式对其作出反应的能力，可能在体内发育过程中引导它们到达合适的靶标方面发挥作用。在本研究中，我们检测了交感神经节前神经元与运动神经元、背根神经节神经元和交感神经节神经元相互作用时的体外生长情况。所有这些细胞类型都可能被正在生长的节前轴突所遇到。交感神经节前生长锥与每种细胞类型的相互作用是不同的。交感神经节前生长锥在运动神经元轴突上形成束状，在与背根神经节神经元的细胞体和轴突接触后塌陷，并且跨越交感神经节神经元的细胞体和轴突生长。这些细胞类型特异性反应与据报道在体外中枢和外周神经元接触后最常见的生长锥行为——塌陷和回缩形成对比，这表明接触介导的识别可能足以实现向合适靶标的生长和相互作用。
