Department of Molecular Biology, College of Life Sciences, Sejong University , Gunja-dong, Gwangjin-gu, Seoul 143-747, Republic of Korea.
J Proteome Res. 2013 Nov 1;12(11):4652-69. doi: 10.1021/pr400689j. Epub 2013 Sep 17.
Diverse abiotic and biotic stresses have marked effects on plant growth and productivity. To combat such stresses, plants have evolved complex but not well understood responses. Common effects upon perception of environmental stress are differential expression of the plant proteome and the synthesis of novel regulatory proteins for protection from and acclimation to stress conditions. Plants respond differently in terms of activation of stress-responsive signaling pathways depending upon the type and nature of the stresses to which they are exposed. Progress in proteomics and systems biology approaches has made it possible to identify the novel proteins and their interactions that function in abiotic stress responses. This will enable elucidation of the functions of individual proteins and their roles in signaling networks. Proteomic analysis of the responses to various stress conditions is performed most commonly using 2D gel electrophoresis and high-throughput identification by LC-MS/MS. Because of recent developments in proteomics techniques, numerous proteomics studies of rice under abiotic stress conditions have been performed. In this review, proteomics studies addressing rice responses to the major environmental stresses--including cold, heat, drought, salt, heavy metals, minerals, UV radiation, and ozone--are discussed. Unique or common protein responses to these stress conditions are summarized and interpreted according to their possible physiological responses in each stress. Additionally, proteomics studies on various plant systems under various abiotic stress conditions are compared to provide deeper understanding of specific and common proteome responses in rice and other plant systems, which will further contribute to the identification of abiotic stress tolerance factor at protein level. Functional analysis of stress-responsive proteins will provide new research objectives with the aim of achieving stable crop productivity in the face of the increasing abiotic stress conditions caused by global climate change.
非生物和生物胁迫对植物的生长和生产力有显著影响。为了应对这些胁迫，植物已经进化出了复杂但尚未被充分理解的反应。常见的环境胁迫感知效应是植物蛋白质组的差异表达和新型调节蛋白的合成，以保护植物免受和适应胁迫条件。植物根据所暴露的胁迫类型和性质，在应激响应信号通路的激活方面表现出不同的反应。蛋白质组学和系统生物学方法的进展使得鉴定在非生物胁迫响应中起作用的新型蛋白质及其相互作用成为可能。这将使我们能够阐明单个蛋白质的功能及其在信号网络中的作用。最常用的 2D 凝胶电泳和 LC-MS/MS 高通量鉴定来进行各种胁迫条件下的蛋白质组分析。由于蛋白质组学技术的最新进展，已经对水稻在非生物胁迫条件下的蛋白质组学研究进行了大量研究。在这篇综述中，讨论了涉及水稻对主要环境胁迫（包括冷、热、干旱、盐、重金属、矿物质、UV 辐射和臭氧）的蛋白质组学研究。根据每种胁迫下可能的生理反应，总结和解释了这些胁迫条件下的独特或常见蛋白质反应。此外，还比较了不同非生物胁迫条件下各种植物系统的蛋白质组学研究，以深入了解水稻和其他植物系统中特定和常见的蛋白质组反应，这将有助于在蛋白质水平上鉴定非生物胁迫耐受性因子。应激响应蛋白的功能分析将提供新的研究目标，旨在实现稳定的作物生产力，以应对全球气候变化引起的日益增加的非生物胁迫。
