Department of Gastroenterology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin Road II, Shanghai 200025, China.
Eur J Med Res. 2013 Aug 28;18(1):28. doi: 10.1186/2047-783X-18-28.
It has been demonstrated that urokinase-type plasminogen activator (uPA) is involved in tumor cell metastasis by degrading the extracellular matrix. However, there is little direct evidence of clinical uPA system expression in peritoneal metastatic tissues of gastric cancer. The objective of this study was to investigate uPA system expression in peritoneal tissues of peritoneal and nonperitoneal metastasis patients, and to explore the diagnostic value of the uPA system.
Expressions of uPA, uPAR, and PAI-1 were measured by semi-quantitative RT-PCR and ELISA. uPA activity was detected using a uPA activity kit.
There was no significant difference in uPA, uPAR, and PAI-1 expression in two types of peritoneal tissue in seven patients with peritoneal metastasis. However, uPA, uPAR, and PAI-1 expressions in peritoneal metastatic lesions were significantly higher than those in normal peritoneal tissues of 24 nonperitoneal metastasis patients (P <0.05). Moreover, no statistical discrepancy of uPA activity was observed in various different tissues.
The expression of the uPA system positively correlates with peritoneal metastasis of gastric cancer. This expression difference in peritoneal or nonperitoneal metastasis patients may provide a reference for diagnosis of peritoneal metastasis.
已证实尿激酶型纤溶酶原激活物（uPA）通过降解细胞外基质参与肿瘤细胞转移。然而，胃癌腹膜转移组织中临床 uPA 系统表达的直接证据很少。本研究旨在探讨腹膜和非腹膜转移患者腹膜组织中 uPA 系统的表达，并探讨 uPA 系统的诊断价值。
采用半定量 RT-PCR 和 ELISA 检测 uPA、uPAR 和 PAI-1 的表达，使用 uPA 活性试剂盒检测 uPA 活性。
7 例腹膜转移患者两种类型腹膜组织中 uPA、uPAR 和 PAI-1 的表达无显著差异。然而，腹膜转移病灶中 uPA、uPAR 和 PAI-1 的表达明显高于 24 例非腹膜转移患者的正常腹膜组织（P<0.05）。此外，各种不同组织中 uPA 活性无统计学差异。
uPA 系统的表达与胃癌腹膜转移呈正相关。腹膜或非腹膜转移患者的这种表达差异可能为腹膜转移的诊断提供参考。
