[叶酸-磁性纳米粒子-基质金属蛋白酶-9反义寡核苷酸复合物的构建及其叶酸分子靶向能力的研究]

[FA-MNP-MMP-9-ASODN complex construction and the study of its FA molecular targeting ability].

作者信息

Liu Tao, Xie Minqiang, Ma Dong, Xu Yiming, Zhang Hongzheng, Zhang Tao

机构信息

Department of Otolaryngology Head and Neck Surgery, People's Hospital of Jiangxi Province, Nanchang, 330006, China.

出版信息

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2013 Jun;27(11):593-7.

PMID:23987010

Abstract

OBJECTIVE

To construct FA targeted magnetic nanocomplex (FA-MNP-MMP-9-ASODN) loading matrix metalloproteinase 9 (MMP-9) antisense oligonucleotide (ASODN) and evaluate its targeting capacity and efficiency of gene transfection to folate receptor (FR) positive NPC.

METHOD

FA-MNP-MMP-9-ASODN was constructed by MMP-9-ASODN coupling with FA-MNP prepared by our research team through the aldehyde-ammonia condensation reaction. To analyze the feasibility of ASODN coupling with nanocarrier agarose gel electrophoresis. Two kinds of HNE-1 and CNE-2 cells and implanted tumors phagocytosis of FA-MNP-MMP-9-ASODN were observed by MRI on tumor-bearing nude mice, iron staining and TEM. To analyze gene transfection of the vector by observing FITC in the cell.

RESULT

The electrophoresis results revealed ASODN successfully coupling with FA-MNP. HNE-1 cell can effectively ingest the nanocomposite,with more FITC in the cell, but CNE-2 cell had not uptake for the nanocomposite, with no FITC in the cell. By comparing with CNE-2 tumor, HNE-1 tumor also can efficiently swallow the nanocomposite.

CONCLUSION

FA-MNP-MMP-9-ASODN nanocomplex is constructed successfully with good FA targeting ability and gene transfection.

摘要

目的

构建负载基质金属蛋白酶9（MMP-9）反义寡核苷酸（ASODN）的叶酸靶向磁性纳米复合物（FA-MNP-MMP-9-ASODN），并评估其对叶酸受体（FR）阳性鼻咽癌的靶向能力和基因转染效率。

方法

通过醛氨缩合反应，将MMP-9-ASODN与本研究团队制备的FA-MNP偶联，构建FA-MNP-MMP-9-ASODN。采用琼脂糖凝胶电泳分析ASODN与纳米载体偶联的可行性。通过磁共振成像（MRI）、铁染色和透射电子显微镜（TEM）观察荷瘤裸鼠体内两种人鼻咽癌细胞系（HNE-1和CNE-2）及移植瘤对FA-MNP-MMP-9-ASODN的吞噬情况。通过观察细胞内的异硫氰酸荧光素（FITC）分析载体的基因转染情况。