Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-911 Vila Real, Portugal.
In Vivo. 2013 Sep-Oct;27(5):635-40.
To study the effect of nebivolol on liver fibrosis induced by common bile duct ligation (BDL) in rats.
After BDL, Wistar rats were divided into three groups (n=24): SO, sham-operated animals; BDL, BDL rats without treatment; BDL+N, BDL rats treated with nebivolol for five weeks. Alanine aminotransferase, aspartate aminotransferase, gamma glutamyltransferase, total bilirubin and albumin levels were assessed. Liver samples collected were stained with hematoxylin-eosin, Masson's trichrom and reticulin.
Mortality reached 37.5% in the BDL group, whereas no deaths were observed in the SO and BDL+N groups. The BDL group showed hepatic damage as evidenced by elevation in serum biochemical parameters and fibrosis scores. These pathophysiological changes were attenuated in the BDL+N group. However, there was no significant difference between these two groups.
Nebivolol improved the survival rate of animals with BDL, but was unable to significantly improve liver function or reduce liver fibrosis.
研究比索洛尔对胆总管结扎（BDL）诱导的大鼠肝纤维化的影响。
BDL 后，Wistar 大鼠分为三组（n=24）：SO，假手术动物；BDL，未治疗的 BDL 大鼠；BDL+N，用比索洛尔治疗五周的 BDL 大鼠。检测丙氨酸氨基转移酶、天冬氨酸氨基转移酶、γ-谷氨酰转移酶、总胆红素和白蛋白水平。收集的肝组织样本用苏木精-伊红、马松三色和网状染色。
BDL 组死亡率达到 37.5%，而 SO 组和 BDL+N 组无死亡。BDL 组血清生化参数和纤维化评分升高，表明存在肝损伤。BDL+N 组减轻了这些病理生理变化。然而，两组之间没有显著差异。
比索洛尔提高了 BDL 动物的存活率，但不能显著改善肝功能或减少肝纤维化。
